NB300-527 Heat Shock Protein 70 (HSP70) antibody

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Mouse anti Human, Mouse Heat Shock Protein 70 (HSP70) JG1

Product Description for Heat Shock Protein 70 (HSP70)

Mouse anti Human, Mouse Heat Shock Protein 70 (HSP70) JG1.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat Shock Protein 70 (HSP70)

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms HSP-70, HSP70-1, HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat Shock 70 kDa Protein 1
Presentation Ascites
Reactivity Hu, Ms
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone JG1
Host Mouse
Isotype IgG3
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide: GSGSSGTGEQKEDQKEEKQ, corresponding to amino acids 615-633 of Mouse mitochondrial Hsp70.
Add. information Research Areas: Cellular Markers
Application Immunocytochemistry, immunoprecipitation, Western Blot, Immunocytochemistry: use at a dilution of 1:50. Western Blot: use at a dilution of 1:500. Immunoprecipitation: assay dependent. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user.By Western blot, this antibody detects a single ~75 kDa band representing mtHSP 70 from U2OS cell homogenate.Immunocytochemical staining of mtHSP 70 in DAP.3 cells with results in a worm-like staining pattern, consistent with mitochondrial localization., immunoprecipitation , Western Blot , Immunocytochemistry
Background The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72 kDa protein which is induced exclusively under stress conditions; HSC 70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP 78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP 70 (mtHSP 70, GRP 75 or mortalin) a 75 kDa protein that is found within the mitochondria. mtHSP 70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP 70 and other inner membrane proteins of the mitochondria.
General Readings This product has been used in:Syken J et al. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions. Proc Natl Acad Sci U S A 96:8499-504 (1999). PubMed PMID: 10411904
Green JM et al. Generation and characterization of monoclonal antibodies specific for members of the mammalian 70-kDa heat shock protein family. Hybridoma 14:347-54 (1995). PubMed PMID: 8522346
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer System:
PBS with 0.05% sodium azide
Detects mitochondrial heat shock protein 70 kDa (mtHSP 70).
Human, Mouse

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