TA326360 Heat shock protein 70 / HSP70 antibody

Mouse monoclonal Hsp70 Antibody

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0.1 mg / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Amphibian, Chicken, Drosophila, Fish, Human, Mouse, Rabbit, Rat, Saccharomyces cerevisiae Heat shock protein 70 / HSP70 3A3

Product Description for Heat shock protein 70 / HSP70

Mouse anti Amphibian, Chicken, Drosophila, Fish, Human, Mouse, Rabbit, Rat, Saccharomyces cerevisiae Heat shock protein 70 / HSP70 3A3.
Presentation: Aff - Purified
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Heat shock protein 70 / HSP70

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms HSP70-1/HSP70-2, HSP70.1, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B
Presentation Aff - Purified
Reactivity Amph, Chk, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 3A3
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P08107
Immunogen:
Human recombinant Hsp70 over expressed in E. coli
GeneID:
3303
Application Western blot: 1-5000 (ECL). 
Immunoprecipitation: 1-2 µg. 
Immunocytochemistry: 1-500. 
Immunohistochemistry on Paraffin Sections: 2.5 µg/ml (Heat-induced antigen retrieval is required).
Background Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4).
All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Concentration 1.0 mg/ml
General Readings
  1. Balashova N. et al. (2005) J Biol Chem 280:2186-96. 
  2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol.38 (1): 1-17. 
  3. Rothman J. (1989) Cell 59: 591 -601. 
  4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 
  5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 
  6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein G affinity Chromatography
Buffer System:
PBS pH 7.2, 50% Glycerol
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat, Amphibian, Chicken, Fish, Saccharomyces cerevisiae, Fruit Fly, Artemia franciscana
Specificity
Specificity:
This antibody detects several members of the heat shock protein 70kDa gene family including Hsp70, Hsc70, p75 and following heat shock, Hsp72 from Yeast, Drosophila, Fish, Mouse, Avian, Amphibian and Human samples.

Accessory Products

Proteins and/or Positive Controls

Proteins for Heat shock protein 70 / HSP70 (24 products)

Catalog No. Species Pres. Purity   Source  

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Purified > 90 % pure as determined by SDS-PAGE analysis. E. coli
2x0.1 mg / €790.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Purified > 90 % pure as determined by SDS-PAGE analysis. E. coli
0.1 mg / €455.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Purified > 90 % pure as determined by SDS-PAGE analysis. E. coli
50 µg / €350.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % pure as determined by SDS-PAGE analysis E. coli
2x0.1 mg / €790.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % pure as determined by SDS-PAGE analysis E. coli
0.1 mg / €455.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (active)

Heat shock protein 70 / HSP70 Human Aff - Purified > 95 % pure as determined by SDS-PAGE analysis E. coli
50 µg / €350.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (1-641, His-tag)

Heat shock protein 70 / HSP70 Human Purified > 95 % pure by SDS PAGE E. coli
0.5 mg / €600.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70 (1-641, His-tag)

Heat shock protein 70 / HSP70 Human Purified > 95 % pure by SDS PAGE E. coli
0.1 mg / €250.00
  OriGene Technologies GmbH

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human in vitro transl.
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

Heat shock protein 70 / HSP70 Human
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18356] - Dna K, 17.7kDa (163aa) with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18357] - Dna K, 69kDa (638aa) with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18358] - Dna K, 41.6kDa (384aa) with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18359] - Dna K, 27.7kDa (255aa) with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18360] - Dna K, 14.6kDa (132aa) with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70

SDS-Page: Hsp70 Protein [NBC1-18367] - HSP70, 72.2kDa (661aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for Heat shock protein 70 / HSP70 (1 products)

Catalog No. Species Pres. Purity   Source  

Hsp70 Lysate

Western Blot: Hsp70 Lysate [NBL1-11749] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for HSPA1A
  Novus Biologicals Inc.
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