NBP1-00582 HEF-1/Cas-L antibody

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Mouse anti Human, Mouse, Rat HEF-1/Cas-L 2G9


Product Description for HEF-1/Cas-L

Mouse anti Human, Mouse, Rat HEF-1/Cas-L 2G9.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for HEF-1/Cas-L

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms anti-CAS L antibody, anti-CASL antibody, anti-Cas like docking antibody, anti-Crk associated substrate related protein antibody, anti-Enhancer of filamentation 1 antibody, anti-HEF 1 antibody, anti-NEDD 9 antibody, anti-NEDD9 antibody, anti-NEDD9 protein antibody, anti-NY REN 12 antigen antibody, anti-Neural cell expressed developmentally down regulated 9 antibody, anti-Neural precursor cell expressed developmentally down regulated 9 antibody, anti-Protein NEDD9 antibody, anti-dJ49G10.2 antibody, anti-dJ761I2.1 antibody, anti-p105 antibody
Presentation Purified
Reactivity Hu, Ms, Rt
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 2G9
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application immunofluorescence, immunohistochemistry, immunoprecipitation, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and Western Blot, immunohistochemistry , immunofluorescence 1:500, immunoprecipitation 1:1000, Western Blot 1:2000-1:6000,
Background HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases; two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that over expression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
General Readings Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.- O'Neill GM ( Molecular and Cellular Biology,Aug 2001)
The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions - Sharmilla D. Bargon et al ( Biochimica et Biophysica Acta (BBA) - Molecular Cell Research,15 December 2005)
The Hematopoietic Isoform of Cas-Hef1-Associated Signal Transducer Regulates Chemokine-Induced Inside-Out Signaling and T Cell Trafficking - Adam G. Regelmann et al ( Immunity,December 2006)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.1% Sodium Azide
protein G purified
Human, mouse, rat HEF-1. Does not recognize p130Cas. Have not tested on Sin1. This mAb mostly detects HEF1 localized at the focal adhesion sites
Human, Mouse, Rat

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