NBP1-00583 HEF-1/ Cas-L antibody

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Mouse anti Human HEF-1/ Cas-L 14A11


Product Description for HEF-1/ Cas-L

Mouse anti Human HEF-1/ Cas-L 14A11.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for HEF-1/ Cas-L

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms anti-CAS L antibody, anti-CASL antibody, anti-Cas like docking antibody, anti-Crk associated substrate related protein antibody, anti-Enhancer of filamentation 1 antibody, anti-HEF 1 antibody, anti-NEDD 9 antibody, anti-NEDD9 antibody, anti-NEDD9 protein antibody, anti-NY REN 12 antigen antibody, anti-Neural cell expressed developmentally down regulated 9 antibody, anti-Neural precursor cell expressed developmentally down regulated 9 antibody, anti-Protein NEDD9 antibody, anti-dJ49G10.2 antibody, anti-dJ761I2.1 antibody, anti-p105 antibody
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP
Clonality Monoclonal
Clone 14A11
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

GST-HEF-1 (82-398aa.)
Isotype control AM03110PU-N, SM12P
Application immunofluorescence, immunoprecipitation, Immunofluorescence and Immunoprecipitation, immunofluorescence 1:100, immunoprecipitation 1ug of antibody per 200ug,
Background HEF1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases; two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that over expression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.
General Readings Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics.- O'Neill GM ( Molecular and Cellular Biology,Aug 2001)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
protein G purified
Buffer System:
0.1% Sodium Azide
protein G purified
Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. It does not recognize p130Cas. Have not tested it on Sin1.

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