AM09224HR-N Helicobacter pylori (CagA Protein) antibody

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Mouse anti Helicobacter pylori 3C10


Product Description for Helicobacter pylori

Mouse anti Helicobacter pylori 3C10.
Properties: (CagA Protein)
Presentation: HRP
Product is tested for ELISA (detection), Enzyme Immunoassay.

Properties for Helicobacter pylori

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms H. pylori
Presentation HRP
Applications E, E(detection)
Clonality Monoclonal
Clone 3C10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

A highly immunogenic 32 KD CagA fragment, recombinant.
Property CagA Protein
Application ELISA: Clone 3C10 can be used as Detection antibody. In Sandwich ELISA, anti-HP CagA clone 10E9 and anti-HP CagA clone 5H10 coated wells match with HRP conjugated clone 3C10 to detect the recombinant antigen. HRP conjugated 3C10 also selectively detected cell lysate of a CagA containing HP strain when clone 10E9 was used as Coating antibody.
Background Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1- 2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Storage Upon receipt, store (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
0.01M PBS, pH 7.2 with 50% Glycerol
Liquid purified IgG fraction
Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml
This antibody is raised against the H. pylori cytotoxin associated gene A protein (CagA).
Reacts with the 32Kd recombinant Helicobacter pylori CagA.

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