AP32828PU-T Helicobacter pylori antibody

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Rabbit anti Helicobacter pylori Helicobacter pylori


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  • AP32828PU-T

Product Description for Helicobacter pylori

Rabbit anti Helicobacter pylori Helicobacter pylori.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry, Paraffin Sections.

Properties for Helicobacter pylori

Product Category Primary Antibodies
Quantity 20 µg
Synonyms H. pylori
Presentation Purified
Reactivity H. pylori
Applications F, ICC/IF, P
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Total sonicate of Helicobacter pylori.
Application Flow Cytometry: 0.5-1 µg/106 cells. 
Immunofluorescence: 1-2 µg/ml.
Immunohistochemistry on Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Helicobacter pylori infected stomach biopsy.
Background Helicobacter pylori (H.pylori) is a gram-negative, microaerophil bacterium found in the stomach. Although Helicobacter pylori is present in the upper gastrointestinal track of more than 50% of the world's population, about 80% of people infected with Helicobacter pylori are asymptomatic. However, Helicobacter pylori is also strongly linked to disease and is thought to have evolved to penetrate the mucoid lining of the stomach. Penetrating the mucosal layer can cause considerable damage to mucosa, and chronic infection can result in or contribute to chronic gastritis, gastric ulcers as well as duodenal ulcers.
There is also strong evidence that Helicobacter pylori plays a causal role in the development of some stomach cancers, likely through chronic infection. However, Helicobacter pylori is also thought to play a role in normal stomach homeostasis which could help explain the large percent of people who have some level of Helicobacter pylori in their gut.
Concentration 0.2 mg/ml
General Readings
  1. Fiorentino M, Ding H, Blanchard TG, Czinn SJ, Sztein MB, Fasano A. Helicobacter pylori-induced disruption of monolayer permeability and proinflammatory cytokine secretion in polarized human gastric epithelial cells. Infect Immun. 2013 Mar;81(3):876-83. doi: 10.1128/IAI.01406-12. Epub 2013 Jan 7. PubMed PMID: 23297384. (Free PMC Article available)
  2. Moodley Y, Linz B, Bond RP, Nieuwoudt M, Soodyall H, Schlebusch CM, et al. Age of the association between Helicobacter pylori and man. PLoS Pathog. 2012;8(5):e1002693. doi: 10.1371/journal.ppat.1002693. Epub 2012 May 10. PubMed PMID: 22589724. (Free PMC Article available, 8 images available)
Storage Store undiluted at 2-8 °C.
Shelf life: one year from despatch.
Protein A Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Serum
This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach. 
The antibody can be used as an ancillary tool for identifying Helicobacter pylori by Immunohistochemistry of tissue sections and may help determine if disease is linked to active infection.
Cellular Localization: Cell surface and cytoplasm.

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