R1093B Hexokinase-1 antibody

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Rabbit anti Yeast Hexokinase-1


Product Description for Hexokinase-1

Rabbit anti Yeast Hexokinase-1.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Hexokinase-1

Product Category Primary Antibodies
Quantity 10 mg
Synonyms Brain form hexokinase, HK1, Hexokinase type I
Presentation Biotin
Reactivity Ye
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Hexokinase [Yeast].
Application Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hexokinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin (RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
Western Blot: 1/500 - 1/2,000.
Background Hexokinase is an allosteric enzyme inhibited by its product glucose-6-phosphate. It is the first step of several metabolic pathways.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
HRP conjugated antibody is cited in:
  1. Eliyahu E, Pnueli L, Melamed D, Scherrer T, Gerber AP, Pines O, et al. Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner. Mol Cell Biol. 2010 Jan;30(1):284-94. doi: 10.1128/MCB.00651-09. Epub . PubMed PMID: 19858288. (Free PMC Article available, 7 images available)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
After reconstitution store undiluted antibody at 2-8 °C for one month or (in aliquots) at -20 °C for long term. Centrifuge product if not completely clear after standing at room temperature.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Multi-step process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Lyophilized purified Ig fraction.
Restore with 1.0 ml of deionized water (or equivalent).
This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
Cross reactivity against Hexokinase from other sources may occur but have not been specifically determined.
Gene ID 2542634

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