discontinued

R1093HRP Hexokinase-1 antibody

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20 mg / €3,300.00

Quick Overview

Rabbit anti Yeast Hexokinase-1

R1093HRP

Product Description for Hexokinase-1

Rabbit anti Yeast Hexokinase-1.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Hexokinase-1

Product Category Primary Antibodies
Quantity 20 mg
Synonyms Brain form hexokinase, HK1, Hexokinase type I
Presentation HRP
Reactivity Ye
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P04807
Immunogen:
Hexokinase [Yeast]
GeneID:
852639
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions: This product has been assayed against 1.0 ug of Hexokinase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:800 to 1:3,000 of the reconstitution concentration is suggested for this product.
Caution Do Not Use Sodium Azide as Preservative.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Eliyahu E, Pnueli L, Melamed D, Scherrer T, Gerber AP, Pines O, et al. Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner. Mol Cell Biol. 2010 Jan;30(1):284-94. doi: 10.1128/MCB.00651-09. Epub . PubMed PMID: 19858288. (Free PMC Article available, 7 images available)
General Readings Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Multi-step process
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 2.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].
Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.
Gene ID 2542634

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