NB100-105 HIF1A / HIF1 alpha antibody

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0.1 ml / €410.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Bovine, Ferret, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep HIF1A / HIF1 alpha H1alpha67

NB100-105

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  • NB100-105

Product Description for HIF1A / HIF1 alpha

Mouse anti Bovine, Ferret, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep HIF1A / HIF1 alpha H1alpha67.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for HIF1A / HIF1 alpha

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Presentation Purified
Reactivity Bov, Ft, Hu, Mky, Ms, Por, Rb, Rt, Sh
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone H1alpha67
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16665
Immunogen:
Fusion protein containing amino acids 432-528 of human HIF-1alpha.
GeneID:
3091
Add. information Research Areas: Hypoxia, Cancer
Application chromatin immunoprecipitation, Immunocytochemistry, immunofluorescence, immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, By Western blot, this antibody recognizes bands at 120 kDa representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1alpha. This antibody has been used to immunoprecipitate human HIF-1 alpha. For ChIP, see Kong, et al., chromatin immunoprecipitation , immunohistochemistry 1:25, immunofluorescence , immunoprecipitation Assay Dependent, Western Blot 1:500, Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen 1:100
Background Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Concentration 4 mg/ml
Storage Store at 4C. Do not freeze.
Format
Purification:
protein G purified
Buffer System:
0.05% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
This is specific for HIF-1 alpha.
Species:
Bovine, Ferret, Hamster, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep
Gene ID 3091

Accessory Products

Proteins and/or Positive Controls

Proteins for HIF1A / HIF1 alpha (17 products)

Catalog No. Species Pres. Purity   Source  

HIF1A / HIF1 alpha (576-785, His-tag)

HIF1A / HIF1 alpha Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (576-785, His-tag)

HIF1A / HIF1 alpha Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (1-85, His-tag)

HIF1A / HIF1 alpha Human Purified > 80 % by SDS – PAGE E. coli
50 µg / €750.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (1-85, His-tag)

HIF1A / HIF1 alpha Human Purified > 80 % by SDS – PAGE E. coli
10 µg / €300.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (530-826)

HIF1A / HIF1 alpha Human Purified > 95 % E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (530-826)

HIF1A / HIF1 alpha Human Purified > 95 % E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
2 µg / €300.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human
10 µg / €380.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Protein
0.1 mg / €310.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
10 µg / €380.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
50 µg / €870.00
  Novus Biologicals Inc.

Hypoxia-inducible factor 1 alpha

Hypoxia-inducible factor 1 alpha Protein
0.2 mg / €400.00
  Novus Biologicals Inc.

Positive controls for HIF1A / HIF1 alpha (2 products)

Catalog No. Species Pres. Purity   Source  

HIF1A 293T Cell Transient Overexpression Lysate(Denatured)

HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

HIF1A overexpression lysate

HIF1A overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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