NB100-479 HIF1A / HIF1 alpha antibody

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0.1 ml / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Mouse HIF1A / HIF1 alpha

NB100-479

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  • NB100-479
  • NB100-479
  • NB100-479

Product Description for HIF1A / HIF1 alpha

Rabbit anti Mouse HIF1A / HIF1 alpha.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for HIF1A / HIF1 alpha

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms ARNT-interacting protein, BHLHE78, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, HIF-1 alpha, Hypoxia-inducible factor 1 alpha, Member of PAS protein 1
Presentation Aff - Purified
Reactivity Ms
Applications C, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16665
Immunogen:
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
GeneID:
3091
Add. information Research Areas: Hypoxia, Cancer
Application immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, This antibody has been tested in Western blot, recognizing a band in hypoxic samples at ~115 kDa. It has only been tested against mouse, rat, human and primate protein. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. This antibody is not recommended for immunoprecipitation. It has also been used for immunohistochemistry (see procedure guide)., immunohistochemistry 5-10 ug/ml, Western Blot 1:500-1:2000, Immunohistochemistry-Paraffin 5-10 ug/ml, 000
Background Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Concentration 1 mg/ml
Storage Store at 4°C. Do Not Freeze.
Format
Purification:
Immunogen affinity purified
Preservatives:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
This is specific for mouse HIF-1 alpha.
Species:
Human, Mouse, Primate, Rat
Gene ID 3091

Accessory Products

Proteins and/or Positive Controls

Proteins for HIF1A / HIF1 alpha (17 products)

Catalog No. Species Pres. Purity   Source  

HIF1A / HIF1 alpha (576-785, His-tag)

HIF1A / HIF1 alpha Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (576-785, His-tag)

HIF1A / HIF1 alpha Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (1-85, His-tag)

HIF1A / HIF1 alpha Human Purified > 80 % by SDS – PAGE E. coli
50 µg / €750.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (1-85, His-tag)

HIF1A / HIF1 alpha Human Purified > 80 % by SDS – PAGE E. coli
10 µg / €300.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (530-826)

HIF1A / HIF1 alpha Human Purified > 95 % E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha (530-826)

HIF1A / HIF1 alpha Human Purified > 95 % E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
2 µg / €300.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human
10 µg / €380.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Protein
0.1 mg / €310.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
10 µg / €380.00
  Novus Biologicals Inc.

HIF1A / HIF1 alpha

HIF1A / HIF1 alpha Human Purified
50 µg / €870.00
  Novus Biologicals Inc.

Hypoxia-inducible factor 1 alpha

Hypoxia-inducible factor 1 alpha Protein
0.2 mg / €400.00
  Novus Biologicals Inc.

Positive controls for HIF1A / HIF1 alpha (2 products)

Catalog No. Species Pres. Purity   Source  

HIF1A 293T Cell Transient Overexpression Lysate(Denatured)

HIF1A 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

HIF1A overexpression lysate

HIF1A overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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