AP08110PU-S His-Tag antibody

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Rabbit anti Mammalia His-Tag

Product Description for His-Tag

Rabbit anti Mammalia His-Tag.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for His-Tag

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity All, Mam
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

The antiserum was produced by immunizing Rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
Application Western Blot (1/500-1/1000).
Concentration 1.0 mg/ml
General Readings
  1. Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533.
  2. Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143.
  3. Els Conrath, K., et al. ( 2001) J. Biol. Chem. 276: 7346-7350.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Immunoaffinity Chromatography using epitope-specific peptide.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Liquid purified Ig fraction.
Aff - Purified
His-Tag Polyclonal Antibody AP08110PU-N detects His-tagged proteins overexpressed in cells.
The antibody recognizes the His-tag fused to either the amino- or carboxy- terminus of targeted proteins in transfected mammalian cells.

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