EUD2601 Histidine decarboxylase antibody

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50 µl / €540.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Canine, Guinea Pig, Human, Mouse, Rat Histidine decarboxylase

EUD2601

Product Description for Histidine decarboxylase

Rabbit anti Canine, Guinea Pig, Human, Mouse, Rat Histidine decarboxylase.
Presentation: Serum
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Histidine decarboxylase

Product Category Primary Antibodies
Target Category
Quantity 50 µl
Synonyms HDC
Presentation Serum
Reactivity Can, GP, Hu, Ms, Rt
Applications C, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P19113
Immunogen:
Recombinant Histidine Decarboxylase produced in E.coli.
GeneID:
3067
Application Immunocytochemistry: 1/1000-1/1500 with overnight incubation at 2-8°C.
Immunohistochemistry on Frozen and Paraffin Embedded Tissues.
Positive Control: Stefanini-fixed frozen sections of Rat fundus.
Western blot: The antiserum detects the 54 kDa and 73 kDa forms in addition to a 63 kDa form (Rat stomach - See Dartsch et al., 1998).
Background Histidine decarboxylase(HDC) is the enzyme that calalyses the conversion of histidine into histamine. It can be found in the histamine secreting ECL cells of some species, as well as in the mast cells.The biogenic amine histamine is an important modulator of numerous physiological processes, including neurotransmission, gastric acid secretion and smooth muscle tone.
General Readings
  1. Dartsch C, Sundler F, Persson L. Antisera against rat recombinant histidine decarboxylase: immunocytochemical studies in different species. Histochem J. 1999 Aug;31(8):507-14. PubMed PMID: 10507457.
  2. Dartsch C, Chen D, Håkanson R, Persson L. Histidine decarboxylase in rat stomach ECL cells: relationship between enzyme activity and different molecular forms. Regul Pept. 1999 May 31;81(1-3):41-8. PubMed PMID: 10395406.
  3. Dartsch C, Chen D, Persson L. Multiple forms of rat stomach histidine decarboxylase may reflect posttranslational activation of the enzyme. Regul Pept. 1998 Oct 16;77(1-3):33-41. PubMed PMID: 9809794.
  4. Dartsch C, Persson L. Recombinant expression of rat histidine decarboxylase: generation of antibodies useful for western blot analysis. Int J Biochem Cell Biol. 1998 Jul;30(7):773-82. PubMed PMID: 9722982.
  5. Lindström E, Björkqvist M, Boketoft A, Chen D, Zhao CM, Kimura K, et al. Neurohormonal regulation of histamine and pancreastatin secretion from isolated rat stomach ECL cells. Regul Pept. 1997 Aug 15;71(2):73-86. PubMed PMID: 9416989.
  6. Zhao CM, Jacobsson G, Chen D, Hâkanson R, Meister B. Exocytotic proteins in enterochromaffin-like (ECL) cells of the rat stomach. Cell Tissue Res. 1997 Dec;290(3):539-51. PubMed PMID: 9369530.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Undiluted Lyophilized Serum
Serum
Reconstitution:
Dissolve the antiserum in 50-100 µl distilled water and dilute further in 0.1M PBS with 1% BSA.
Species Reactivity
Species reactivity (tested):
Human, Rat, Mouse, Canine (Dog) and Guinea Pig.
Specificity
Specificity:
This antibody reacts with HDC.
Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
Gene ID 3067

Accessory Products

Proteins and/or Positive Controls

Proteins for Histidine decarboxylase (1 products)

Catalog No. Species Pres. Purity   Source  

Histidine decarboxylase

Histidine decarboxylase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Positive controls for Histidine decarboxylase (1 products)

Catalog No. Species Pres. Purity   Source  

HDC overexpression lysate

HDC overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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