AP31734SU-N Histone H2A/H4 pSer1 antibody

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50 µl / €470.00
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Quick Overview

Rabbit anti Human Histone H2A/H4

Product Description for Histone H2A/H4

Rabbit anti Human Histone H2A/H4.
Properties: pSer1
Presentation: Serum
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Histone H2A/H4

Product Category Primary Antibodies
Quantity 50 µl
Synonyms H2A Histone Family
Presentation Serum
Reactivity Hu
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
kLH-conjugated, synthetic peptide corresponding to amino acids 1-9 of Human Histone H2A ([pS]GRGKQGGK-C), with a C-terminal cysteine added to facilitate conjugation.
The immunizing sequence has 8/9 amino acid identity with Histone H4.
Property pSer1
Application Western Blot (1/2000-1/10000): Detects phosphorylated Histone H2A/H4 (Ser1) in 10 µg of acid-extracted proteins from colcemid-arrested HeLa cells. Acid-extracted proteins from untreated HeLa cells and from colcemid-arrested HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with AP31734SU-N (1/5000).
Immunohistochemistry on Paraffin Sections (1/100).
Background Histones are the core component of nucleosome. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones including phosphorylation.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Antiserum containing 0.05% Sodium Azide and 38% Glycerol.
Serum
Species Reactivity
Species reactivity (expected):
Broad species cross-reactivity expected due to sequence conservation.
Species reactivity (tested):
Human.
Specificity
Specificity:
Recognizes phosphorylated Histones H2A and H4, Mr 16kD and 10kD respectively.

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