AM33078PU-S Histone H3 pSer28 antibody

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25 µg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rat anti Human Histone H3 HTA28

AM33078PU-S

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  • AM33078PU-S

Product Description for Histone H3

Rat anti Human Histone H3 HTA28.
Properties: pSer28
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Flow Cytometry, Western blot / Immunoblot.

Properties for Histone H3

Product Category Primary Antibodies
Quantity 25 µg
Synonyms H3 Histone family, HIST1H3
Presentation Purified
Reactivity Hu
Applications F, ICC/IF, IP, WB
Clonality Monoclonal
Clone HTA28
Host Rat
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.
Property pSer28
Isotype control SM15P, SM15PX
Application Western blot: Each lot of this antibody is quality control tested.
Recommended Dilutions: Use 5 µg/ml antibody per 5 ml antibody dilution buffer for each mini-gel.
Mass Cytometry.
Immunofluorescence
(Reported).
Immunoprecipitation 
(Reported).
Intracellular Flow Cytometry
 (Reported).
Background H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. 
Structure: H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution: Nucleus.
Function: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Regulation: H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Interaction: Two molecules of H3 form a heterotetramer with two molecules of H4.
Concentration 0.5 mg/ml
General Readings
  1. Hirata A, Inada K, Tsukamoto T, Sakai H, Mizoshita T, Yanai T, et al. Characterization of a monoclonal antibody, HTA28, recognizing a histone H3 phosphorylation site as a useful marker of M-phase cells. J Histochem Cytochem. 2004 Nov;52(11):1503-9. PubMed PMID: 15505345.
  2. Goto H, Tomono Y, Ajiro K, Kosako H, Fujita M, Sakurai M, et al. Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation. J Biol Chem. 1999 Sep 3;274(36):25543-9. PubMed PMID: 10464286.
  3. Ozawa K. Reduction of phosphorylated histone H3 serine 10 and serine 28 cell cycle marker intensities after DNA damage. Cytometry A. 2008 Jun;73(6):517-27. doi: 10.1002/cyto.a.20559. PubMed PMID: 18395832.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
The HTA28 monoclonal antibody recognizes Histone H3-Phosphorylated (Ser28).
Species:
Human.

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