TA328045 Histone H3 pSer10 antibody

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0.1 mg / €325.00
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Mouse anti Human Histone H3 11D8

TA328045

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  • TA328045

Product Description for Histone H3

Mouse anti Human Histone H3 11D8.
Properties: pSer10
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Histone H3

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms H3 Histone family, HIST1H3
Presentation Aff - Purified
Reactivity Hu
Applications ICC/IF, WB
Clonality Monoclonal
Clone 11D8
Host Mouse
Isotype IgG2b
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Immunogen:
Modified synthetic peptide conjugated to KLH
Property pSer10
Application Western blot: Each lot of this antibody is quality control tested.
Recommended Dilutions: Use 1 µg per ml antibody dilution buffer for each mini-gel.
Immunofluorescence.
Background H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. 
Structure: H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution: Nucleus.
Function: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Regulation: H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Interaction: Two molecules of H3 form a heterotetramer with two molecules of H4.
Concentration 0.5 mg/ml
General Readings
  1. Choi HS, Choi BY, Cho YY, Zhu F, Bode AM, Dong Z. Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha. J Biol Chem. 2005 Apr 8;280(14):13545-53. Epub 2005 Jan 31. PubMed PMID: 15684425.
  2. Goto H, Yasui Y, Nigg EA, Inagaki M. Aurora-B phosphorylates Histone H3 at serine28 with regard to the mitotic chromosome condensation. Genes Cells. 2002 Jan;7(1):11-7. PubMed PMID: 11856369.
  3. Garcia BA, Barber CM, Hake SB, Ptak C, Turner FB, Busby SA, et al. Modifications of human histone H3 variants during mitosis. Biochemistry. 2005 Oct 4;44(39):13202-13. PubMed PMID: 16185088.
  4. Hans F, Dimitrov S. Histone H3 phosphorylation and cell division. Oncogene. 2001 May 28;20(24):3021-7. PubMed PMID: 11420717.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Antigen Affinity Chromatography
Buffer System:
PBS, pH 7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purified IgG fraction
Aff - Purified
Specificity
Specificity:
The 11D8 monoclonal antibody recognizes Human Histone H3-Phosphorylated (Ser10).

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