SP2143 Histone H4 (acetyl K5) antibody

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0.1 ml / €740.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Amphibian, Drosophila, Mammalia, Plant, Yeast Histone H4

SP2143

Product Description for Histone H4

Rabbit anti Amphibian, Drosophila, Mammalia, Plant, Yeast Histone H4.
Properties: (acetyl K5)
Presentation: Serum
Product is tested for ChIP assay, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Histone H4

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms H4/A, H4FA, HIST1H4
Presentation Serum
Reactivity Amph, Dros, Mam, Pl, Ye
Applications ChIP, E, ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P62805
Immunogen:
Ovalbumin-conjugated peptide.
AA Sequence:
NSGRGAcKGGKGLCc
GeneID:
121504
Property acetyl K5
Application ELISA: 1/1000.
Western Blot: 1/600.
Immunofluorescence: 1/400.
Immunoprecipitation.
Chromatin Immunoprecipitation (ChIP).
Background Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.
General Readings
  1. Munks RJ, Moore J, O'Neill LP, Turner BM. Histone H4 acetylation in Drosophila. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies. FEBS Lett. 1991 Jun 24;284(2):245-8. PubMed PMID: 2060643.
  2. Turner BM, Birley AJ, Lavender J. Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei. Cell. 1992 Apr 17;69(2):375-84. PubMed PMID: 1568251.
  3. Belyaev N, Keohane AM, Turner BM. Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells. Hum Genet. 1996 May;97(5):573-8. PubMed PMID: 8655133.
  4. Auger A, Galarneau L, Altaf M, Nourani A, Doyon Y, Utley RT, et al. Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants. Mol Cell Biol. 2008 Apr;28(7):2257-70. doi: 10.1128/MCB.01755-07. Epub 2008 Jan 22. PubMed PMID: 18212047. (Free PMC Article available, 6 images available)
  5. Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases. J Inflamm (Lond). 2011 Jan 27;8(1):1. doi: 10.1186/1476-9255-8-1. PubMed PMID: 21272292. (Free PMC Article available, 5 images available)
  6. Ito K, Barnes PJ, Adcock IM. Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12. Mol Cell Biol. 2000 Sep;20(18):6891-903. PubMed PMID: 10958685. (Free PMC Article available, 9 images available)
  7. Nie M, Pang L, Inoue H, Knox AJ. Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation. Mol Cell Biol. 2003 Dec;23(24):9233-44. PubMed PMID: 14645533. (Free PMC Article available, 8 images available)
  8. Nie M, Knox AJ, Pang L. beta2-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation. J Immunol. 2005 Jul 1;175(1):478-86. PubMed PMID: 15972682.
  9. Tanny JC, Kirkpatrick DS, Gerber SA, Gygi SP, Moazed D. Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions. Mol Cell Biol. 2004 Aug;24(16):6931-46. PubMed PMID: 15282295. (Free PMC Article available, 9 images available)
  10. Spada F, Chioda M, Thompson EM. Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles. J Cell Biochem. 2005 Aug 1;95(5):885-901. PubMed PMID: 15937898.
  11. Lacoste N, Utley RT, Hunter JM, Poirier GG, Côte J. Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase. J Biol Chem. 2002 Aug 23;277(34):30421-4. Epub 2002 Jul 3. PubMed PMID: 12097318.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid Serum containing 0.09% Sodium Azide as preservative.
Serum
Specificity
Specificity:
This antibody is specific for Histone H4 acetylated at Lysine 5.
Species:
Drosophila, Yeast, Mammals, Plants, Amphibia.
Gene ID 121504

Accessory Products

Proteins and/or Positive Controls

Proteins for Histone H4 (20 products)

Catalog No. Species Pres. Purity   Source  

HIST1H4J

HIST1H4J Human Purified
  Abnova Taiwan Corp.

HIST1H4K

HIST1H4K Human Purified
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Histone H4 (full length, N-term HIS tag)

Histone H4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Histone H4 (full length, N-term HIS tag)

Histone H4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Histone H4 (N-term HIS tag)

Histone H4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Histone H4 (full length, N-term HIS tag)

Histone H4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Histone H4 (full length, N-term HIS tag)

Histone H4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Histone H4

Histone H4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Histone H4 (2 products)

Catalog No. Species Pres. Purity   Source  

HIST1H4I Lysate

Western Blot: HIST1H4I Lysate [NBL1-11569] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for HIST1H4I
  Novus Biologicals Inc.

Histone H4 Lysate

Western Blot: Histone H4 Lysate [NBL1-11576] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for HIST4H4
  Novus Biologicals Inc.
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