AM05229PU-N HIV reverse transcriptase (HIV-RT) antibody

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0.1 mg / €430.00
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Quick Overview

Mouse anti HIV reverse transcriptase (HIV-RT) AS34


Product Description for HIV reverse transcriptase (HIV-RT)

Mouse anti HIV reverse transcriptase (HIV-RT) AS34.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation.

Properties for HIV reverse transcriptase (HIV-RT)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Applications E, IP
Clonality Monoclonal
Clone AS34
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Recombinant Immuno-Deficiency Virus reverse transcriptase (HIV-RT).
Isotype control AM03095PU-N
Add. information Antibody Source: In vitro cell culture.
Application ELISA: This antibody has been used as a coating antibody at 10 µg/ml in a two site ELISA with clone AS35 (Cat.-No AM05230BT-N).
Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of < 1 ng/ml HIV-RT. Recommended sample volumes are 50-200 ml.
Immunoaffinity Purification: This antibody, covalently coupled to protein A Agarose, has been used to Immunoaffinity purify HIV-RT.
Immunoprecipitation: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). Also accomplished using Protein G Agarose.
Background Anti-Human Immunodeficiency Virus Reverse Transcriptase is an antibody meant to target the Reverse Transcriptase (RT) efficiency in HIV infections. RT allows the viral RNA to be transcribed to host DNA and synthesize new DNA composed of the virus’ genome. Because RT also exhibits RNaseH activity, it enables the production of a DNA/RNA hybrid used for synthesis. Additionally, it can cause errors during transcription, creating difficulties in isolating the virus and promoting further infection.
Concentration 1 mg/ml
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Protein A/G Chromatography.
Buffer System:
Dulbecco's PBS (without Ca2+ and Mg2+)
0.08% Sodium Azide
Liquid (0.2µm sterile filtered) purified IgG fraction (>95% pure by SDS-PAGE).
This antibody recognizes an epitope on HIV-RT that is different from the recognized epitope of clone AS35 (Cat.-No AM05230BT-N).

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