DDX0250P-50 HLA-alpha1 antibody

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50 µg / €330.00
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Mouse anti Human HLA-alpha1 mAb90

DDX0250P-50

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  • DDX0250P-50

Product Description for HLA-alpha1

Mouse anti Human HLA-alpha1 mAb90.
Presentation: Purified
Product is tested for Enzyme Immunoassay, ELISA (capture), Functional assay, Flow Cytometry.

Properties for HLA-alpha1

Product Category Primary Antibodies
Quantity 50 µg
Synonyms HLA Class I Alpha-1 Domain, HLA class I histocompatibility antigen A-1 alpha chain, HLAA
Presentation Purified
Reactivity Hu
Applications E, E(capture), F, FN
Clonality Monoclonal
Clone mAb90
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P30443
Immunogen:
Tonsillar B cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application FACS, cell culture, ELISA capture (detecting antibody DDX0270). 
Usage recommendation:
*This monoclonal antibody may be used between 1-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Antibodies to HLA class I α1 domain trigger apoptosis of activated T-cells and also control the growth/survival of human B lymphocytes. Addition of mAb 90 strongly inhibits the proliferation of CD40- activated total tonsillar B cells, purified naive, germinal center and memory B-cell subsets. mAb 90 binds to an epitope of the α-1 domain of HLA class I heavy chain induces apoptotic cell death of activated but not resting peripheral T lymphocytes.
(Genestier L. et al., 1997 Blood, 90, 726-35; Genestier L. et al. 1997, Blood, 90, 3629-3639).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Genestier L, Meffre G, Garrone P, Pin JJ, Liu YJ, Banchereau J, et al. Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes. Blood. 1997 Jul 15;90(2):726-35. PubMed PMID: 9226173.
  2. Genestier L, Prigent AF, Paillot R, Quemeneur L, Durand I, Banchereau J, et al. Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis. J Biol Chem. 1998 Feb 27;273(9):5060-6. PubMed PMID: 9478956.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography.
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
State:
Purified

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