SM2012P HLA Class I ABC Xenograft marker antibody

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0.2 mg / €470.00
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Rat anti Human HLA Class I ABC YTH862.2

SM2012P

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  • SM2012P

Product Description for HLA Class I ABC

Rat anti Human HLA Class I ABC YTH862.2.
Properties: Xenograft marker
Presentation: Purified
Product is tested for Frozen Sections, Functional assay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Flow Cytometry.

Properties for HLA Class I ABC

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I
Presentation Purified
Reactivity Hu
Applications C, F, FN, ICC/IF, IP
Clonality Monoclonal
Clone YTH862.2
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
PHA activated Human peripheral blood lymphocytes. 
Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Property Xenograft marker
Isotype control SM19P
Application Immunoprecipitation.
Immunofluorescence.

Functional Assays: Removal of Sodium Azide by dialysis is recommended prior to use. 
Immunohistochemistry on Frozen Sections.
Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Background The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Concentration 1.0 mg/ml
General Readings
  1. Genestier L, Paillot R, Bonnefoy-Berard N, Meffre G, Flacher M, Fèvre D, et al. Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain. Blood. 1997 Nov 1;90(9):3629-39. PubMed PMID: 9345047.
  2. Genestier L, Meffre G, Garrone P, Pin JJ, Liu YJ, Banchereau J, et al. Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes. Blood. 1997 Jul 15;90(2):726-35. PubMed PMID: 9226173.
  3. Genestier L, Prigent AF, Paillot R, Quemeneur L, Durand I, Banchereau J, et al. Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis. J Biol Chem. 1998 Feb 27;273(9):5060-6. PubMed PMID: 9478956.
  4. Liu S, Tian Y, Chlenski A, Yang Q, Zage P, Salwen HR, et al. Cross-talk between Schwann cells and neuroblasts influences the biology of neuroblastoma xenografts. Am J Pathol. 2005 Mar;166(3):891-900. PubMed PMID: 15743800. (Free PMC Article available, 5 images available)
  5. Schmidt J, Barthel K, Wrede A, Salajegheh M, Bähr M, Dalakas MC. Interrelation of inflammation and APP in sIBM: IL-1 beta induces accumulation of beta-amyloid in skeletal muscle. Brain. 2008 May;131(Pt 5):1228-40. doi: 10.1093/brain/awn053. Epub 2008 Apr 17. PubMed PMID: 18420712. (Free PMC Article available, 6 images available)
  6. Muth IE, Barthel K, Bähr M, Dalakas MC, Schmidt J. Proinflammatory cell stress in sporadic inclusion body myositis muscle: overexpression of alphaB-crystallin is associated with amyloid precursor protein and accumulation of beta-amyloid. J Neurol Neurosurg Psychiatry. 2009 Dec;80(12):1344-9. doi: 10.1136/jnnp.2009.174276. Epub 2009 May 25. PubMed PMID: 19470495.
  7. Schmidt J, Rakocevic G, Raju R, Dalakas MC. Upregulated inducible co-stimulator (ICOS) and ICOS-ligand in inclusion body myositis muscle: significance for CD8+ T cell cytotoxicity. Brain. 2004 May;127(Pt 5):1182-90. Epub 2004 Mar 26. PubMed PMID: 15047591.
  8. Pluchinotta, M. balancing act between stratification and EMT in cultured human thymic epithelial cells. Thesis 2016. http://infoscience.epfl.ch/record/218089/files/EPFL_TH6942.pdf
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction from Tissue Culture Supernatant
Purified
Specificity
Specificity:
This antibody recognizes a monomorphic determinant on the Human HLA Class I heavy chain.
Epitope analysis has shown that this antibody binds in the alpha-1 domain.
Antibody SM2012P detects the Human HLA class I ABC antigen without cross-reactivity to Mouse.
Studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (See Genestier, L. et al. for details). 
Negative Species: Mouse.
Species:
Human.

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