AM26699AF-N HLA class II DM alpha / HLA-DMA antibody

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0.1 mg / €200.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse HLA class II DM alpha / HLA-DMA M5/114

AM26699AF-N

Product Description for HLA class II DM alpha / HLA-DMA

Rat anti Mouse HLA class II DM alpha / HLA-DMA M5/114.
Presentation: Azide Free
Product is tested for Functional assay, Paraffin Sections, Frozen Sections, Western blot / Immunoblot, Immunoprecipitation, Flow Cytometry.

Properties for HLA class II DM alpha / HLA-DMA

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DM alpha chain, DMA, HLA class II histocompatibility antigen, MHC class II antigen DMA, RING6, Really interesting new gene 6 protein
Presentation Azide Free
Reactivity Ms
Applications C, F, FN, IP, P, WB
Clonality Monoclonal
Clone M5/114
Host Rat
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P28067
Immunogen:
Activated C57BL/6 mouse spleen cells
GeneID:
3108
Isotype control SM19A
Application Flow Cytometry: 2 μg/ml.
Immunoprecipitation.
Western blot.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections.
Functional Application: Blocking of T cell proliferative responses.
Background MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Concentration 1.0 mg/ml
General Readings
  1. Bhattacharya A, Dorf ME, Springer TA. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127(6):2488-95. PubMed PMID: 6170707.
  2. Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, et al. Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 26;72(4):635-48. PubMed PMID: 7679955.
  3. De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC. Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct;108 ( Pt 10):3219-31. PubMed PMID: 7593283.
  4. Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, et al. Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1;155(1):53-63. PubMed PMID: 11581285. (Free PMC Article available, 8 images available)
  5. Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY. Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1;174(3):1205-12. PubMed PMID: 15661874.
  6. Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, et al. CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug;19(8):977-92. PubMed PMID: 17804692.
  7. Zang W, Kalache S, Lin M, Schroppel B, Murphy B. MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec;16(12):3661-8. Epub 2005 Oct 12. PubMed PMID: 16221866.
  8. Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, et al. Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8(2):143-55. PubMed PMID: 9491996.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein-G Affinity Chromatography
Buffer System:
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Azide Free
Species Reactivity
Species reactivity (tested):
Mouse
Specificity
Specificity:

The antibody reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. 

Gene ID 3108

Accessory Products

Proteins and/or Positive Controls

Proteins for HLA class II DM alpha / HLA-DMA (2 products)

Catalog No. Species Pres. Purity   Source  

HLA class II DM alpha / HLA-DMA

HLA class II DM alpha / HLA-DMA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

HLA class II DM alpha / HLA-DMA

HLA class II DM alpha / HLA-DMA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for HLA class II DM alpha / HLA-DMA (2 products)

Catalog No. Species Pres. Purity   Source  

HLA-DMA 293T Cell Transient Overexpression Lysate(Denatured)

HLA-DMA 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

HLA DMA Lysate

Western Blot: HLA DMA Lysate [NBL1-11591] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for HLA
  Novus Biologicals Inc.
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