DM3105 HLA Class II DP antibody

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Mouse anti Human HLA Class II DP Bra-FB6


Product Description for HLA Class II DP

Mouse anti Human HLA Class II DP Bra-FB6.
Presentation: Supernatant
Product is tested for Frozen Sections.

Properties for HLA Class II DP

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Supernatant
Reactivity Hu
Applications C
Clonality Monoclonal
Clone Bra-FB6
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

REH (Human ALL cell line)
Application Immunohistochemistry on frozen tissues (1:50, Acetone-fixed only !) Recommended positive control: Human tonsil Other applications not tested.
(According to customer's report this antibody works in flow cytometry at a 1:150 - 1:300 dilution). Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.3 mg/ml
General Readings
  1. Poláková K, Chorváth B, Sedlák J, Duraj J, Matoska J, Karpatová M. Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line. Neoplasma. 1985;32(6):641-8. PubMed PMID: 3911080.
  2. Chorváth B, Duraj J, Sedlák J, Plesková I, Munozová H, Buc M. Supplementary characteristics of anti-MHC class II monoclonal antibodies elicited by an ALL cell line: immunofluorescence cytofluorometry, C-dependent cytotoxicity, two-dimensional analysis of antigen. Neoplasma. 1987;34(4):417-25. PubMed PMID: 3309687.
  3. Tissue Antigen
Storage Refrigerate at 4°C. Do not freeze. Shelf life: one year from despatch.
Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability. Withdraw aliquots as needed with a micropipette and keep
concentrated stock at 4°C. In general, the 0.05M borate, pH 8.0, containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies.
Buffer System:
20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Liquid supernatant
This antibody recognizes 26-34 kD class II major histocompatibility complex, MHC differentiated monocytes and B cells.

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