discontinued

NB500-579 HLA class II DR beta 1 / HLA-DRB1 antibody

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Search for all "HLA class II DR beta 1 / HLA-DRB1"

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Mouse anti Human HLA class II DR beta 1 / HLA-DRB1 MEM-267

Product Description for HLA class II DR beta 1 / HLA-DRB1

Mouse anti Human HLA class II DR beta 1 / HLA-DRB1 MEM-267.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for HLA class II DR beta 1 / HLA-DRB1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms DR-1, DR1, DRB1-1 beta chain, HLA class II histocompatibility antigen, MHC class II antigen DRB1
Presentation Purified
Reactivity Hu
Applications E, F, WB
Clonality Monoclonal
Clone MEM-267
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P04229
Immunogen:
Purified, insoluble Human DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies
Isotype control AM03110PU-N, SM12P
Add. information Research Areas: Lipid and Metabolism, Immunology
Application ELISA, flow cytometry / FACS analysis, Western Blot, ELISA, Flow cytometry / FACS analysis and Western Blot In FACS The antibody stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. In WB predicted molecular weight 26-28 kDa. , flow cytometry / FACS analysis assay dependent , ELISA assay dependent, Western Blot assay dependent
Background HLA DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of an alpha chain (36 kDa) and a beta chain (27 kDa). It is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T helper cells. They therefore have a critical role in the initiation of the immune response.
Concentration 1 mg/ml
General Readings Carven GJ et al. Monoclonal antibodies specific for the empty conformation of HLA-DR1 reveal aspects of the conformational change associated with peptide binding. J Biol Chem 279:16561-70 (2004). PubMed: 14757758
Search PubMed (MEDLINE) for references to HLA DR
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Protein A purified
Buffer System:
15mM Sodium Azide
Purity:
Protein A purified
State:
Purified
Specificity
Specificity:
The MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1.
Species:
Human

Accessory Products

Proteins and/or Positive Controls

Proteins for HLA class II DR beta 1 / HLA-DRB1 (2 products)

Catalog No. Species Pres. Purity   Source  

HLA class II DR beta 1 / HLA-DRB1 (30-227, His-tag)

HLA class II DR beta 1 / HLA-DRB1 Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

HLA class II DR beta 1 / HLA-DRB1 (30-227, His-tag)

HLA class II DR beta 1 / HLA-DRB1 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH
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