DDX9021A647-100 HPA-5b antibody

See related secondary antibodies

Search for all "HPA-5b"

0.1 mg / €730.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Human anti Human HPA-5b F2H11

DDX9021A647-100

More Views

  • DDX9021A647-100
  • DDX9021A647-100
  • DDX9021A647-100

Product Description for HPA-5b

Human anti Human HPA-5b F2H11.
Presentation: Alexa Fluor 647
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Properties for HPA-5b

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms human platelet antigen 5b
Presentation Alexa Fluor 647
Reactivity Hu
Applications F, ICC/IF
Clonality Monoclonal
Clone F2H11
Host Human
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P17301
Immunogen:
CD40 activated and EBV immortalized human B lymphocytes
GeneID:
3673
Application Platelet phenotyping by flow cytometry, IF, ELISA, platelet aggregation studies.
Usage recommendation:
*This monoclonal antibody may be used between 5-25μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Neonatal AlloImmune Thrombocytopenia (NAIT) is the commonest cause of severe isolated thrombocytopenia in the foetus and newborn. In Caucasians, 97% of NAIT are caused by HPA-1a or HPA- 5b alloantibodies. B4F12 was isolated after combination of CD40 activation and Epstein-Barr virus transformation of B cells isolated from a woman with high titre of anti -HPA-5b alloantibodies. B4F12 human monoclonal antibody recognizes GPIa-IIa preferentially on activated human platelets (Mérieux Y, Rigal D, Pin JJ, XVIIth Regional Congress, International Society of Blood Transfusion, Madrid 2007 ; Guillot-Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. HPA5b Abstract Singapour 2007.
  2. Guillot-Chene P, Lebecque S, Rigal D. [Towards an industrial control of the cloning of lymphocytes B human for the manufacturing of monoclonal antibodies stemming from the human repertoire]. Ann Pharm Fr. 2009 May;67(3):182-6. doi: 10.1016/j.pharma.2009.02.007. Epub 2009 Apr 8. PubMed PMID: 19446667.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 647
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
Specificity
Specificity:
GPIa-IIa preferentially on acitvated platelets.
Species:
Human.

Accessory Products

Proteins and/or Positive Controls

Proteins for HPA-5b (2 products)

Catalog No. Species Pres. Purity   Source  

CD49b / ITGA2

CD49b / ITGA2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CD49b / ITGA2

CD49b / ITGA2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for HPA-5b (2 products)

Catalog No. Species Pres. Purity   Source  

Integrin alpha 2 Lysate

Western Blot: Integrin alpha 2 Lysate [NBL1-12062] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for ITGA2
  Novus Biologicals Inc.

ITGA2 overexpression lysate

ITGA2 overexpression lysate
  OriGene Technologies, Inc.
  • LinkedIn