NB110-17178 HPV16 + HPV18 E7 Protein antibody

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0.2 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Virus HPV16 + HPV18 E7 Protein 716-281

NB110-17178

Product Description for HPV16 + HPV18 E7 Protein

Mouse anti Virus HPV16 + HPV18 E7 Protein 716-281.
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for HPV16 + HPV18 E7 Protein

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms HPV16 E7, HPV18 E7, Human Papilloma Virus Early protein 7
Reactivity Vi
Applications E, WB
Clonality Monoclonal
Clone 716-281
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Full size recombinant E7 protein of HPV type 16
Add. information Research Areas: Immunology
Application ELISA, Western Blot, ELISA and Western Blot Suitable for sandwich immunoassay. Predicted molecular weight 11 kDa. , ELISA Assay dependent, Western Blot Assay dependent
Background The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (of, for example, types 16 and 18) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (of, for example, type 6). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
Concentration 2 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.1% Sodium Azide
Purity:
protein G purified
Specificity
Specificity:
Recognizes the E7 protein of HPV type 16. Cross-reacts with E7 protein of HPV type 18.
Species:
Virus

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