AM20059PU-S HSP22 antibody

See related secondary antibodies

Search for all "HSP22"

Quick Overview

Mouse anti Human, Mouse, Rat HSP22 3C12-H11


More Views

  • AM20059PU-S

Product Description for HSP22

Mouse anti Human, Mouse, Rat HSP22 3C12-H11.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for HSP22

Product Category Primary Antibodies
Quantity 25 µg
Synonyms Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, HMN2, HSB8, Heat shock 22kDa protein 8, HspB8
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, E, WB
Clonality Monoclonal
Clone 3C12-H11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
His-tagged Human recombinant HSP22
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA.
Western blot (1/2000).
1 µg/mL was sufficient for detection of Hsp22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Background Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27.
Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity.
In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy (4).
Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).
Concentration 1.0 mg/ml
General Readings
  1. Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6.
  2. Benndorf R., et al. (2001) J Biol Chem 276: 26753-26761.
  3. Sun X., et al. (2004) J Biol Chem 279: 2394-2402.
  4. Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652.
  5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G Chromatography.
Buffer System:
PBS, pH 7.4 containing 50% Glycerol as stabilizer and 0.09% Sodium Azide as preservative.
Liquid purified IgG fraction.
Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric (weak) forms in Western Blot.
Does not cross react with alpha Crystalline.
Exogenous HSP22 detected in dimeric form.
Gene ID 2030

Accessory Products

Proteins and/or Positive Controls

Proteins for HSP22 (2 products)

Catalog No. Species Pres. Purity   Source  


SLC29A1 Human
  Abnova Taiwan Corp.


SLC29A1 Human
  Abnova Taiwan Corp.
  • LinkedIn