TA326394 HSP22 antibody

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0.1 mg / €325.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human, Mouse, Rat HSP22 3C12-H11

TA326394

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  • TA326394

Product Description for HSP22

Mouse anti Human, Mouse, Rat HSP22 3C12-H11.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for HSP22

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, HMN2, HSB8, Heat shock 22kDa protein 8, HspB8
Presentation Purified
Reactivity Hu, Ms, Rt
Applications C, E, WB
Clonality Monoclonal
Clone 3C12-H11
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UJY2
Immunogen:
His-tagged Human recombinant HSP22
GeneID:
2030
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA.
Western blot (1/2000).
1 µg/mL was sufficient for detection of Hsp22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Immunohistochemistry.
Background Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27.
Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity.
In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy (4).
Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).
Concentration 1.0 mg/ml
General Readings
  1. Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1-6.
  2. Benndorf R., et al. (2001) J Biol Chem 276: 26753-26761.
  3. Sun X., et al. (2004) J Biol Chem 279: 2394-2402.
  4. Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652.
  5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography.
Buffer System:
PBS, pH 7.4 containing 50% Glycerol as stabilizer and 0.09% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Purified
Specificity
Specificity:
Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric (weak) forms in Western Blot.
Does not cross react with alpha Crystalline.
Exogenous HSP22 detected in dimeric form.

Accessory Products

Proteins and/or Positive Controls

Proteins for HSP22 (2 products)

Catalog No. Species Pres. Purity   Source  

SLC29A1

SLC29A1 Human
2 µg / €300.00
  Abnova Taiwan Corp.

SLC29A1

SLC29A1 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.
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