AM12025PU-N Hsp40 / Ydj1 antibody

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Mouse anti Yeast Hsp40 / Ydj1 1G10.H8

Product Description for Hsp40 / Ydj1

Mouse anti Yeast Hsp40 / Ydj1 1G10.H8.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Hsp40 / Ydj1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ye
Applications E, IP, WB
Clonality Monoclonal
Clone 1G10.H8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Full length protein yeast HSP40 (YDJ1)
Isotype control AM03095PU-N
Application Western blot: 1/2000.
Background Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not.
Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
Concentration 1.0 mg/ml
General Readings
  1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
  2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.
  3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.
  4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
  5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
  6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.
  7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
0.09 % sodium azide in 50 % glycerol
Liquid Ig fraction
Species Reactivity
Species reactivity (tested):
Yeast. Does not cross react with human, mouse, and rat.
This antibody detects Hsp40 T ~40kDa.

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