TA309369 Hsp40 / Ydj1 antibody

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0.1 mg / €325.00
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Quick Overview

Mouse anti Yeast Hsp40 / Ydj1 1G10.H8

Product Description for Hsp40 / Ydj1

Mouse anti Yeast Hsp40 / Ydj1 1G10.H8.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Hsp40 / Ydj1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Ye
Applications E, IP, WB
Clonality Monoclonal
Clone 1G10.H8
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P39102
Immunogen:
Full length protein yeast HSP40 (YDJ1)
GeneID:
850779
Isotype control AM03095PU-N
Application Western blot: 1/2000.
Immunoprecipitation.
ELISA.
Background Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not.
Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
Concentration 1.0 mg/ml
General Readings
  1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
  2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.
  3. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.
  4. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
  5. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
  6. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.
  7. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.
Storage Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
0.09 % sodium azide in 50 % glycerol
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Yeast. Does not cross react with human, mouse, and rat.
Specificity
Specificity:
This antibody detects Hsp40 T ~40kDa.

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