BB-PA1214 HSP70s antibody

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0.1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat HSP70s

BB-PA1214

Product Description for HSP70s

Rabbit anti Human, Mouse, Rat HSP70s.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for HSP70s

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications C, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Boster Immunoleader
Manufacturer certificates ISO 9001

Datasheet Extract

Immunogen
Swiss Prot Num:
E9PSH5
Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminal of human HSP70s, identical to the related rat and mouse sequence.
Background The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
General Readings
  1. Tavaria M, Gabriele T, Kola I, Anderson RL. A hitchhiker's guide to the human Hsp70 family. Cell Stress Chaperones. 1996 Apr;1(1):23-8. PubMed PMID: 9222585. (Free PMC Article available)
  2. Morano KA. New tricks for an old dog: the evolving world of Hsp70. Ann N Y Acad Sci. 2007 Oct;1113:1-14. Epub 2007 May 18. PubMed PMID: 17513460.
  3. Lüders J, Demand J, Höhfeld J. The ubiquitin-related BAG-1 provides a link between the molecular chaperones Hsc70/Hsp70 and the proteasome. J Biol Chem. 2000 Feb 18;275(7):4613-7. PubMed PMID: 10671488.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Format
Buffer System:
5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
State:
Lyophilized
Aff - Purified
Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

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