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AM26735PU-N Human Kappa Light Chain antibody

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0.1 mg / €310.00
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Quick Overview

Mouse anti Human Human Kappa Light Chain kappa-117

AM26735PU-N

Product Description for Human Kappa Light Chain

Mouse anti Human Human Kappa Light Chain kappa-117.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections.

Properties for Human Kappa Light Chain

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, P
Clonality Monoclonal
Clone kappa-117
Host Mouse
Isotype IgG1
Shipping to USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified human IgG
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry on frozen sections: 0.25 µg/ml (1/800).
Immunohistochemistry on paraffin sections: Has been described to work with proteinase K pretreatment.
Positive control: Human tonsil.
General Readings
  1. Odermatt BF, Knecht H, Hagen MF, Fehr J, Rüttner JR. Diagnostic and prognostic value of monoclonal antibodies in immunophenotyping of T cell lymphomas. Acta Haematol. 1987;77(2):72-7. PubMed PMID: 3111143.
  2. Odermatt BF, Maurer R, Rüttner JR. [Morphologic and immunologic criteria for the diagnosis of non-Hodgkin's lymphoma of low-grade malignancy]. Schweiz Rundsch Med Prax. 1986 Apr 8;75(15):401-7. PubMed PMID: 3485809.
  3. Stein H, Dallenbach F, Dienemann D. [Differentiation pathways of normal and malignant cells of the lymphatic system]. Verh Dtsch Ges Pathol. 1988;72:57-85. PubMed PMID: 3064479.
Storage Store lyophilized at 2-8°C for 6 month or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity purified
Buffer System:
Phosphate buffered saline pH 7.2 (PBS)
Preservatives:
0.02 % sodium azide
Stabilizers:
5 mg/ml bovine serum albumin (BSA)
State:
Lyophilized Ig fraction
Purified
Reconstitution:
Reconstitute by adding 0.5 ml distilled water. This stock solution contains 0. 2 mg/ml IgG.
Specificity
Specificity:
This antibody detects Human Kappa Light Chain. Monoclonal antibody κ-117 can be used for the detection of surface and intracytoplasmic immunoglobulins on normal and neoplastic B-cells. In conjunction with clone λ-1, the light chain isotype of a B-cell population can be determined. Antigen distribution:
Isolated cells: Antigen receptor bearing B-cells are stained positively.
Tissue sections: κ-117 labels follicular and non-follicular B-cells in lymphoid tissues. Intravasal staining can also be observed caused by circulating Ig. Strong labeling occurs with plasma cells in paraffin embedded tissues. The antibody stains surface and intracellular immunoglobulins in cryostat sections. Only intracellular light chains are stained in formaldehyde fixed and paraffin embedded tissues after enzyme digestion (eg. plasma cells are stained in the latter case.)

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