AM33125PU-T Human Kappa Light Chain antibody

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20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Human Kappa Light Chain L1C1

Product Description for Human Kappa Light Chain

Mouse anti Human Human Kappa Light Chain L1C1.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Immunoprecipitation, Flow Cytometry, Frozen Sections, Paraffin Sections.

Properties for Human Kappa Light Chain

Product Category Primary Antibodies
Quantity 20 µg
Presentation Purified
Reactivity Hu
Applications C, F, IP, P, WB
Clonality Monoclonal
Clone L1C1
Host Mouse
Isotype IgG1
Molecular weight ~22.5 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human B-Lymphoma Cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Quantification of immunoglobulins.
For coating, Use Antibody without BSA.  
Flow Cytometry: 0.5-2.0 µg/106 cells. 
Immunoprecipitation: 1-2 µg/500 µg protein lysate. 
Western Blot: 0.5-1 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Background If a lymph node or other tissue of lymphoid origin is normal or benign, it should contain a mixture of lambda and kappa light chain positive cells. However, if there is only one type, such as all kappa light chain positive, then they may have all been derived from a clonal population. This may be indicative of a pathological condition, including a malignancy.  As such, the kappa light chain antibody has been reported to help identify leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The underlying mechanism of identification by the kappa light chain antibody in these various cancers would be their expression of the kappa, but not lambda, light chain. 
Hence, the kappa light chain antibody has an overall usefulness in identifying normal B-cells expressing kappa light chain as well as helping to identify malignancies or potentially other pathologies characterized by a clonally derived kappa light chain positive population. 
Antibody to the kappa light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Concentration 0.2 mg/ml
General Readings
  1. Kochwa S. (1976) Immunoelectrophoresis. In Manual of Clinical Immunology. American Society of Microbiology. P17-35.
  2. Korkolopoulou P, Pangalis GA, Patsouris E, Boussiotis VA, Kittas C. B-cell lymphoma of large multilobated type: an immunohistochemical study of 8 cases and review of the literature. Leuk Lymphoma. 1994 Mar;13(1-2):151-9. PubMed PMID: 8025516.
  3. Abe M, Goto T, Kennel SJ, Wolfenbarger D, Macy SD, Weiss DT, et al. Production and immunodiagnostic applications of antihuman light chain monoclonal antibodies. Am J Clin Pathol. 1993 Jul;100(1):67-74. PubMed PMID: 7688492.
  4. Berg AM, RF Troxler, G Grillone, J Kasznica, K Kane, AS Cohen, M Skinner. Localized amyloidosis of the larynx: evidence for light chain composition. Ann. Otol. Rhinol. Laryngol. 884-889 (1993).
  5. Takahashi H, Fujita S, Okabe H, Tsuda N, Tezuka F. Immunophenotypic analysis of extranodal non-Hodgkin's lymphomas in the oral cavity. Pathol Res Pract. 1993 Apr;189(3):300-11. PubMed PMID: 8332573.
  6. Momose H, Chen YY, Ben-Ezra J, Weiss LM. Nodular lymphocyte-predominant Hodgkin's disease: study of immunoglobulin light chain protein and mRNA expression. Hum Pathol. 1992 Oct;23(10):1115-9. PubMed PMID: 1398641.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
This monoclonal L1C1 antibody reacts with the Kappa Light chain of Human immunoglobulin and shows no cross-reaction with Lambda Light chain or any of the five heavy chains.
The kappa light chain itself is one of the two small polypeptide subunits of an antibody, the other being lambda.
Antibodies are produced by the B-cells in lymphoid tissue. Each B lymphocyte expresses either lambda or kappa light chain but never both together.  Hence, the kappa light chain antibody is a useful marker for identifying B lymphocytes expressing kappa light chain.
The antibody reacts in immunohistology with paraffin embedded tissues and can be used to detect kappa positive normal and malignant B cells. 
Cellular Localization: Cell Surface, Cytoplasmic and Secreted. 
Negative Species: Rat.

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