AM50288PU-T Human Kappa Light Chain antibody

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20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Human Kappa Light Chain HP6053

Product Description for Human Kappa Light Chain

Mouse anti Human Human Kappa Light Chain HP6053.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Human Kappa Light Chain

Product Category Primary Antibodies
Quantity 20 µg
Synonyms HCAK1, IGKC, Ig Kappa Chain C Region, Immunoglobulin KM
Presentation Purified
Reactivity Hu
Applications C, F, IP, P, WB
Clonality Monoclonal
Clone HP6053
Host Mouse
Isotype IgG1
Molecular weight ~22.5kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Purified Human Ig kappa chain.
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry:
 0.5-1 μg/106 cells.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Concentration 0.2 mg/ml
General Readings
  1. Takahashi H, Fujita S, Okabe H, Tsuda N, Tezuka F. Immunophenotypic analysis of extranodal non-Hodgkin's lymphomas in the oral cavity. Pathol Res Pract. 1993 Apr;189(3):300-11. PubMed PMID: 8332573.
  2. Momose H, Chen YY, Ben-Ezra J, Weiss LM. Nodular lymphocyte-predominant Hodgkin's disease: study of immunoglobulin light chain protein and mRNA expression. Hum Pathol. 1992 Oct;23(10):1115-9. PubMed PMID: 1398641.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Cellular Localization: Cell Surface, cytoplasmic and secreted.

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