AM32823PU-S Human Lambda Light Chain antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Human Lambda Light Chain LcN-2


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  • AM32823PU-S

Product Description for Human Lambda Light Chain

Mouse anti Human Human Lambda Light Chain LcN-2.
Presentation: Purified
Product is tested for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Human Lambda Light Chain

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone LcN-2
Host Mouse
Isotype IgG2a
Molecular weight ~22.5 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified Human IgG
Isotype control AM03096PU-N
Application ELISA (Use Antibody without BSA for coating).
Western Blot: 0.5-5 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunofluorescence: 1-2 μg/ml.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunohistochemistry on Frozen and Fixed-Formalin Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer,
pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Background An antibody is a Y-shaped glycoprotein belonging to the immunoglobulin superfamily. Antibodies are produced by B lymphocytes, and utilized by the immune system to identify and neutralize bacteria, viruses and other foreign targets. Two large heavy and two small light chains connected by disulfide bonds comprise the basic structural antibody unit, and form the antibody Y shape.
There are two types of immunoglobulin light chains in mammals, lambda and kappa. Each B lymphocyte expresses only one class, either lambda or kappa. Once determined, the class remains fixed for the life of the B lymphocyte. The total kappa to lambda ratio is approximately 2:1 in serum from a healthy individual, measuring intact whole antibodies and 1:1.5 if measuring free light chains. A highly divergent kappa to lambda ratio can be indicative of a malignancy or inflammatory condition.
Concentration 0.2 mg/ml
General Readings
  1. Development of a highly-sensitive multi-plex assay using monoclonal antibodies for the simultaneous measurement of kappa and lambda immunoglobulin free lightchains in serum and urine. Campbell JP, Cobbold M, Wang Y, Goodall M, Bonney SL, Chamba A, Birtwistle J, Plant T, Afzal Z, Jefferis R, Drayson MT. J Immunol Methods. 2013 May 31;391(1-2):1-13. doi: 10.1016/j.jim.2013.01.014. Epub 2013 Feb 4.
  2. Defining the impact of individual sample variability on routine immunoassay of serum free lightchains (sFLC) in multiple myeloma. Murng SH, Follows L, Whitfield P, Snowden JA, Swallow K, Green K, Sargur R, Egner W. Clin Exp Immunol. 2013 Feb;171(2):201-9. doi: 10.1111/cei.12011.
  3. Normalization of free lightchain kappa/lambda ratio is a robust prognostic indicator of favorable outcome in patients with multiple myeloma. Iwama K, Chihara D, Tsuda K, Ugai T, Sugihara H, Nishida Y, Yamakura M, Takeuchi M, Matsue K. Eur J Haematol. 2013 Feb;90(2):134-41. doi: 10.1111/ejh.12050. Epub 2013 Jan 7.
  4. de Vos M, Druez P, Nicaise M, Ngendahayo P, Sinapi I, Mineur P. Multiple myeloma presenting as hepatic nodular lesion. Acta Clin Belg. 2012 Sep-Oct;67(5):378-80. PubMed PMID: 23189550.
  5. Riveiro-Barciela M, Martínez-Valle F, Vilardell-Tarrés M. Nephrotic syndrome and lambda light-chain monoclonal gammopathy suggestive of primary amyloidosis with positive staining for AA amyloid. J Am Geriatr Soc. 2012 Oct;60(10):1974-5. doi: 10.1111/j.1532-5415.2012.04160.x. PubMed PMID: 23057449.
  6. Redegeld FA, Thio M, Groot Kormelink T. Polyclonal immunoglobulin free light chain and chronic inflammation. Mayo Clin Proc. 2012 Oct;87(10):1032-3; author reply 1033. doi: 10.1016/j.mayocp.2012.07.012. PubMed PMID: 23036675. (Free PMC Article available, 1 images available)
  7. Inability of a monoclonal antilight chain antibody to detect clonal plasma cells in a patient with multiple myeloma by multicolor flow cytometry. van Velzen JF, van den Blink D, Bloem AC. Cytometry B Clin Cytom. 2013 Jan-Feb;84(1):30-2. doi: 10.1002/cyto.b.21044. Epub 2012 Sep 27.
  8. Unsworth DJ, Wallage MJ, Sarkar E, Lock RJ. Abnormalities of serum-free light chain in patients with primary antibody deficiency in the absence of B lymphocyte clonality. J Clin Pathol. 2012 Dec;65(12):1128-31. doi: 10.1136/jclinpath-2012-201044. Epub 2012 Sep 21. PubMed PMID: 23002283.
  9. Campbell JP et. al. J Immunol Methods. 2013;391(1-2):1-13.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Affinity Chromatography on Protein A/G
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
This Monoclonal LcN-2 antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed.
Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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