AM32827PU-S Human Lambda Light Chain antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Human Lambda Light Chain HP6054


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  • AM32827PU-S

Product Description for Human Lambda Light Chain

Mouse anti Human Human Lambda Light Chain HP6054.
Presentation: Purified
Product is tested for Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Flow Cytometry, Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Human Lambda Light Chain

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone HP6054
Host Mouse
Isotype IgG2a
Molecular weight ~22.5 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified Human IgG myeloma proteins covalently coupled to polyaminostyren microbeads.
Application ELISA (Use Antibody without BSA for coating).
Western Blot:
 0.5-1 µg/ml.  
Immunoprecipitation: 1-2 μg/500 μg protein lysate. 
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Immunohistochemistry on Frozen and Fixed-Formalin
 Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer,
pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Background Immunoglobulins (Ig) are important effector molecules of adaptive humoral immune responses. They are basically 'Y' shaped molecule consisting of four polypeptide chains, two identical light chains (23kD) and two identical heavy chains (50-70kD), connected by disulphide bonds. Both the light and heavy chains of the immunoglobulin consist of a constant region and variable region domain. The heavy chains are usually of five types, depending upon which the antibody is classified into five types G, A M D and E, while the light is of two types, lambda and kappa. The two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda, is present per antibody in mammals. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain are present in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L). However with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine.
Concentration 0.2 mg/ml
General Readings
  1.  Reimer et al. Hybridoma 3: 263, 1984.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purigfied IgG fraction from Bioreactor Concentrate
Species Reactivity
Species reactivity (tested):
This Monoclonal antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Cellular Localization: Cell Surface, Cytoplasmic and Secrete.

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