AM50121PU-N Human Lambda Light Chain antibody

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0.2 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Human Lambda Light Chain SPM559

AM50121PU-N

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  • AM50121PU-N

Product Description for Human Lambda Light Chain

Mouse anti Human Human Lambda Light Chain SPM559.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Human Lambda Light Chain

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM559
Host Mouse
Isotype IgG2a
Molecular weight ~22.5kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified human IgG.
Isotype control AM03096PU-N
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30
minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Concentration 0.2 mg/ml
General Readings
  1. Campbell JP et. al. J Immunol Methods. 2013;391(1-2):1-13.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Cellular Localization: Cell surface, cytoplasmic and secreted.
Species:
Human.

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