AP21321AF-N Hyaluronidase antibody

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10 mg / €240.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Sheep Hyaluronidase


Product Description for Hyaluronidase

Rabbit anti Sheep Hyaluronidase.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Hyaluronidase

Product Category Primary Antibodies
Quantity 10 mg
Synonyms HYAL-1, HYAL1, HYAL2, Hyal-2, Hyaluronidase, Hyaluronoglucosaminidase, LUCA-1, LUCA1
Presentation Azide Free
Reactivity Sh
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Hyaluronidase isolated and purified from Sheep testes.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Application This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/4,000).
Background Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis.
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins.
Lyophilized IgG fraction.
Azide Free
Restore by adding 1.0 ml sterile distilled water.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.

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