discontinued

GTX10625 Hypoxia-inducible factor 1 alpha antibody

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1 kit / €520.00

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anti Bovine, Ferret, Human, Mouse, Rabbit, Rat, Sheep Hypoxia-inducible factor 1 alpha

GTX10625

Product Description for Hypoxia-inducible factor 1 alpha

anti Bovine, Ferret, Human, Mouse, Rabbit, Rat, Sheep Hypoxia-inducible factor 1 alpha.
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Hypoxia-inducible factor 1 alpha

Product Category Primary Antibodies
Quantity 1 kit
Reactivity Bov, Ft, Hu, Ms, Rb, Rt, Sh
Applications C, P, WB
Clonality Polyclonal
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Immunogen
Immunogen:
GTX20463 = Fusion protein containing amino acids 432-528 of human HIF-1a. GTX28366 = Fusion protein containing amino acids 329-530 of human HIF-1a.GTX30603 = Fusion protein containing amino acids 432-528 of human HIF-1a. GTX16535 = A synthetic peptide m
Application IHC-Fr, IHC-P, WB
Background HIF1 (hypoxia inducible factor 1) is a heterodimer consisting of an alpha and a beta subunit, both belonging to the basic helix loop helix Per-aryl hydrocarbon receptor nuclear translocatorSim (PAS) family of transcription factors. HIF1 is a key component of a widely operative transcriptional response activated by hypoxia, cobaltous ions, and iron chelation. HIF1 activates transcription of hypoxia-inducible genes, including those encoding: erythropoietin, vascular endothelial growth (VEGF), heme oxygenase 1, inducible nitric oxide synthase,and the glycolytic enzymes aldolase A, enolase 1,lactate dehydrogenase A, phosphofructokinase I, and phosphoglycerate kinase 1. The C-terminal of HIF 1 alpha binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF.
Storage Keep as concentrated solution. Store at 4
Format
Purification:
GTX20463: Protein G purified cell culture supernatant in PBS.GTX28366: Protein G purified mouse ascites in PBS.GTX16535: Antigen affinity purified rabbit antisera in TBS.GTX30603: affinity purified
Buffer System:
GTX20463 and GTX28366: PBS. GTX16535: TBS containing 0.09% BSA and 0.09% sodium azide. Protein conc. is batch dependent. Please refer to product vial. GTX30603: TBS with 0.1% sodium azide.
Purity:
GTX20463: Protein G purified cell culture supernatant in PBS.GTX28366: Protein G purified mouse ascites in PBS.GTX16535: Antigen affinity purified rabbit antisera in TBS.GTX30603: affinity purified
State:
Liquid.This kit contains these following antibody samples:GTX20463= 50 ul purified antibody.GTX28366= 50 ul purified antibody.GTX16535= 25 ul purified antibody.
Specificity
Specificity:
GTX20463 recognizes human, sheep, mouse, rat, porcine, rabbit and ferret HIF-1a. Bovine (-). GTX28366 recognizes human and rat. Mouse testing is in progress. Other species have not been tested.GTX30603 recognizes human and rat. Other species not tested.GTX16535 recognizes human and mouse HIF-1alpha (in WB). Prelimary results with mouse tissue have been positive (for IHC). Used on mouse eye at 1:100 with high pH retrieval.

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