SM7045P I Kappa K iota/epsilon (IKKi/IKKe) antibody

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Mouse anti Human, Mouse I Kappa K iota/epsilon (IKKi/IKKe) 107A1458


Product Description for I Kappa K iota/epsilon (IKKi/IKKe)

Mouse anti Human, Mouse I Kappa K iota/epsilon (IKKi/IKKe) 107A1458.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for I Kappa K iota/epsilon (IKKi/IKKe)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms IkappaK iota/epsilon
Presentation Purified
Reactivity Hu, Ms
Applications WB
Clonality Monoclonal
Clone 107A1458
Host Mouse
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

This antibody was raised against a His-tagged full-length human IKKe protein.
Application Western blot analysis: 2 µg/ml. An 80 kDa band should be observed. This antibody will cross-react with mouse IKKe. Recommended Positive Control: Daudi, HeLa, or NIH 3T3. Other applications note tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. &Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).
  2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).
  3. Didonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandi, E., and Karin, M. Nature 388: 548- (1997).
  4. Regnier, C.H., Ho, Y.S., Gao, X., Goeddel, D.V., Cao, Z., and Rothe, M. Cell 90, 373 (1997).
  5. Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennett, B.L., Li, J., Young, D.B., Barbosa, M., Mann, M., Manning, A., Rao, A. Science 278: 860 (1997).
  6. Shimada T, et al. Int. Immun
Storage Store undiluted at 4°C, stable for 6 months. Do not freeze. Shelf life: one year from despatch.
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Recently, using a substractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKK-i in cells phosphorylates Ser32 and Ser36 of IkBa. Reacts with human and mouse.

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