SP6389P I Kappa K iota/epsilon (IKKi/IKKe) antibody

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Rabbit anti Human I Kappa K iota/epsilon (IKKi/IKKe)


Product Description for I Kappa K iota/epsilon (IKKi/IKKe)

Rabbit anti Human I Kappa K iota/epsilon (IKKi/IKKe).
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for I Kappa K iota/epsilon (IKKi/IKKe)

Product Category Primary Antibodies
Quantity 50 µg
Synonyms IkappaK iota/epsilon
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer AnaSpec

Datasheet Extract

A peptide (NRIIERLNRVPAPPDV) corresponding to amino acids 701 to 716 of human IKKε/IKK-i (1, 2).

Format: This antibody is supplied as liquid immunoaffinity chomatography purified immunglobulin fraction in Phosphate buffered saline with 0.02% sodium azide as preservative.
Application Suitable for Western blot (0.5 - 1 µg/ml, Jurkat whole cell lysate can be used as positive control and an 80 kD band should be detected). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.5 mg/ml
General Readings
  1. Peters RT, Liao SM, Maniatis T. IKKepsilon is part of a novel PMA-inducible IkappaB kinase complex. Mol Cell. 2000 Mar;5(3):513-22. PubMed PMID: 10882136.
  2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, et al. IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int Immunol. 1999 Aug;11(8):1357-62. PubMed PMID: 10421793.
Storage Store the antibody undiluted at 4-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Aff - Purified
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. IκB is phosphorylated by IκB kinase (IKK) complex that contains IKKαď?¬ IKKβď?¬ and IKKγ. A novel molecule in the IKK complex was recently identified and designated IKKε/IKK-i (1, 2). IKKεď?  is required for the activation of NF-κB by PMA and T cell receptors but not by TNFα and IL-1 (1). IKKε/IKK-i message is expressed in a variety of tissues and is inducible by TNFα, IL-1, and LPS (2).
This antibody detects IKKε/IkKι. It has no cross response to IKKαď?¬ IKKβď?¬ď?  or IKKγ.

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