AP31099PU-N IDH1 antibody

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0.1 mg / €340.00
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Quick Overview

Goat anti Yeast IDH1

Product Description for IDH1

Goat anti Yeast IDH1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for IDH1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Isocitrate dehydrogenase [NAD] subunit 1, Isocitric dehydrogenase, NAD(+)-specific ICDH, YNL037C, mitochondrial
Presentation Aff - Purified
Reactivity Ye
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P28834
Immunogen:
Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1.
AA Sequence:
C-EPGSRHVGLDIKGQN
GeneID:
855691
Add. information Calculated Molecular Weight: 39.3kDa (NP_014361.1).
Application Peptide ELISA: Detection Limit: 1/16000.
Western Blot: 0.3-1 µg/ml. A band of ~38kDa is observed in wildtype lysates of S. cerevisiae (Data kindly provided by F. Reggiiori, University Medical Centre Utrecht, Netherlands).
Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Concentration 0.5 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Saccharomyces cerevisiae S288c
Species reactivity (tested):
S.cerevisiae
Specificity
Specificity:
Recognizes IDH1 (Yeast).
Gene ID 216

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