discontinued

AP08683PU-N IFNAR1 pSer535/539 antibody

See related secondary antibodies

Search for all "IFNAR1"

Quick Overview

Rabbit anti Bovine, Canine, Human, Mouse, Rat, Sheep IFNAR1

Product Description for IFNAR1

Rabbit anti Bovine, Canine, Human, Mouse, Rat, Sheep IFNAR1.
Properties: pSer535/539
Presentation: Aff - Purified
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for IFNAR1

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms CRF2-1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, IFN-R-1, IFN-alpha Receptor alpha, IFN-alpha/beta receptor 1, Interferon-alpha/beta receptor alpha chain, Type I interferon receptor 1
Presentation Aff - Purified
Reactivity Bov, Can, Hu, Ms, non-Human Primates, Rt, Sh
Applications C, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17181
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of Human IFNAR1.
GeneID:
3454
Property pSer535/539
Application Western blot: 1/1000.
Immunohistochemistry: 1/1000.
Background Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
Product Citations
Originator or purchased from resellers:
  1. Kumar KG, Krolewski JJ, Fuchs SY. Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor. J Biol Chem. 2004 Nov 5;279(45):46614-20. Epub 2004 Aug 26. PubMed PMID: 15337770.
  2. Bhattacharya S, HuangFu WC, Liu J, Veeranki S, Baker DP, Koumenis C, et al. Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor. J Biol Chem. 2010 Jan 22;285(4):2318-25. doi: 10.1074/jbc.M109.071498. Epub 2009 Nov 30. PubMed PMID: 19948722. (Free PMC Article available, 5 images available)
General Readings
  1. Biron CA. Interferons alpha and beta as immune regulators--a new look. Immunity. 2001 Jun;14(6):661-4. PubMed PMID: 11420036.
  2. Kirkwood J. Cancer immunotherapy: the interferon-alpha experience. Semin Oncol. 2002 Jun;29(3 Suppl 7):18-26. PubMed PMID: 12068384.
  3. Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY. SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor. EMBO J. 2003 Oct 15;22(20):5480-90. PubMed PMID: 14532120. (Free PMC Article available, 7 images available)
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Buffer System:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.
State:
Liquid purified Ig fraction.
Aff - Purified
Species Reactivity
Species reactivity (expected):
Bovine, Canine, Human, Mouse, Sheep and non-Human Primates.
Species reactivity (tested):
Rat.
Specificity
Specificity:
This antibody is specific for the IFNAR1 protein phosphorylated at Ser535,539.
Note: The Molecular Weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110-130k.
Gene ID 3454

Accessory Products

Proteins and/or Positive Controls

Proteins for IFNAR1 (5 products)

Catalog No. Species Pres. Purity   Source  

IFNAR1

IFNAR1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

IFNAR1

IFNAR1 Human Purified
  Abnova Taiwan Corp.

IFNAR1

IFNAR1 Human Purified
  Abnova Taiwan Corp.

IFNAR1

IFNAR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

IFNAR1

IFNAR1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for IFNAR1 (1 products)

Catalog No. Species Pres. Purity   Source  

IFNAR1 293T Cell Transient Overexpression Lysate(Denatured)

IFNAR1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.
  • LinkedIn