TA302937 IFT74 (C-term) antibody

Goat Polyclonal Antibody against IFT74

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0.1 mg / €360.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human, Rat IFT74

Product Description for IFT74

Goat anti Human, Rat IFT74.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for IFT74

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CCDC2, CMG-1, CMG1, Capillary morphogenesis protein 1, Coiled-coil domain-containing protein 2, Intraflagellar transport protein 74 homolog
Presentation Aff - Purified
Reactivity Hu, Rt
Applications E, ICC/IF, P, WB
Clonality Polyclonal
Host Goat
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96LB3
Immunogen:
Peptide from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.
AA Sequence:
C-KTIVDALHSTSGN
GeneID:
80173
Property C-term
Application Peptide ELISA: Detection Limit: 1/16000.
Western blot: 0.3-1 µg/ml. Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Momeni et al, BMC Neurol. 2006 Dec 13;6:44. PMID: 17166276).
Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. 
Immunocytochemistry: Successfully used at 2.5 mg/ml to stain Rat cortical neurons (See picture and PMID: 17166276).
Background Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24% identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.
Concentration 0.5 mg/ml
General Readings 1. Momeni P et al.: Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.
BMC Neurol. 2006 Dec 13;6(1):44. PMID: 17166276
2.Boldt K et.al. Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice. J Clin Invest. 2011 Jun 1;121(6):2169-80. PMID: 21606596
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH 7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Rat and Human.
Specificity
Specificity:
This antibody recognizes IFT74/CCDC2 at C-term.

Accessory Products

Proteins and/or Positive Controls

Proteins for IFT74 (2 products)

Catalog No. Species Pres. Purity   Source  

IFT74

IFT74 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

IFT74

IFT74 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.
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