NB100-75529 IME antibody

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Chicken anti Yeast IME

Product Description for IME

Chicken anti Yeast IME.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for IME

Product Category Primary Antibodies
Quantity 50 µg
Presentation Aff - Purified
Reactivity Ye
Applications WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Swiss Prot Num:
The immunogen for this antibody was a sequence from 97a.a. to 279a.a. from IME(a 360a.a. long protein)
Application Western Blot, Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development., Western Blot
Background Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression.
General Readings 1. Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B., Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,, et al.
Life with 6000 genes
2. Galibert,F., Alexandraki,D., Baur,A., Boles,E., Chalwatzis,N., Chuat,J.C., Coster,F., Cziepluch,C., De Haan,M., Domdey,H.,, et al.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X
3. Saccharomyces Genome Database (yeast-curator@genome.stanford.edu)., et al.
Direct Submission
4. Saccharomyces Genome Database (yeast-curator@genome.stanford.edu)., et al.
Direct Submission
5. , Smith H.E., Su S.S.Y., Neigeborn L., Driscoll S.E., Mitchell A.P.
Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae.
6. , Sherman A., Shefer M., Sagee S., Kassir Y.
Post-transcriptional regulation of IME1 determines initiation of meiosis in Saccharomyces cerevisiae.
7. , Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., et al.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
No Preservative
Immunogen affinity purified
Aff - Purified
Gene ID 853556

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