discontinued

AM20272PU-M Immunoglobulin J chain antibody

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Rabbit anti Human Immunoglobulin J chain SP105

Product Description for Immunoglobulin J chain

Rabbit anti Human Immunoglobulin J chain SP105.
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Immunoglobulin J chain

Product Category Primary Antibodies
Target Category
Quantity 0.5 ml
Synonyms IGCJ, IGJ
Presentation Purified
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone SP105
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01591
Immunogen:
Synthetic peptide near the internal region of Human J-chain.
GeneID:
3512
Isotype control PP501P
Add. information Molecular weight: 17 kDa
Application Western Blot.
Immunohistochemistry on Paraffin Sections: 1/100 for 10 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.
Positive Control: Tonsil.
Background J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM.
Storage Store the antibody undiluted at 2–8°C.
DO NOT FREEZE!
Shelf life: One year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
PBS, pH 7.6 containing 1% BSA as stabilizer and 0.09% Sodium Azide as preservative
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
Recognizes Immunoglobulin J Chain.
Cellular localisation: Cytoplasm.
Species:
Human.
Gene ID 3512

Accessory Products

Proteins and/or Positive Controls

Proteins for Immunoglobulin J chain (5 products)

Catalog No. Species Pres. Purity   Source  

Immunoglobulin J chain

Immunoglobulin J chain Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Immunoglobulin J chain (23-159, His-tag)

Immunoglobulin J chain Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Immunoglobulin J chain (23-159, His-tag)

Immunoglobulin J chain Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Immunoglobulin J chain

Immunoglobulin J chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Immunoglobulin J chain

Immunoglobulin J chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Immunoglobulin J chain (3 products)

Catalog No. Species Pres. Purity   Source  

IGJ 293T Cell Transient Overexpression Lysate(Denatured)

IGJ 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-IGJ antibody (H00003512-B01) by Western Blots.
  Abnova Taiwan Corp.

IgJ Lysate

Western Blot: IgJ Lysate [NBL1-11879] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IGJ
  Novus Biologicals Inc.

IGJ overexpression lysate

IGJ overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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