SM1013P Immunoglobulin J chain antibody

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0.2 mg / €330.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Immunoglobulin J chain MC19-9

SM1013P

Product Description for Immunoglobulin J chain

Mouse anti Human Immunoglobulin J chain MC19-9.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Immunoglobulin J chain

Product Category Primary Antibodies
Target Category
Quantity 0.2 mg
Synonyms IGCJ, IGJ
Presentation Purified
Reactivity Hu
Applications C, E, IP, WB
Clonality Monoclonal
Clone MC19-9
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01591
Immunogen:
Human J Chain.
GeneID:
3512
Remarks:
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA (20 µg/ml).
Immunoprecipitation (20 µg/ml).
Immunohistochemistry on Frozen Sections (1/200).
Western blotting (only under Reducing Conditions).
Background Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.
Concentration 1.0 mg/ml
Product Citations
Purchased from Acris:
  1. Leithäuser F, Bäuerle M, Huynh MQ, Möller P. Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma. Blood. 2001 Nov 1;98(9):2762-70. PubMed PMID: 11675349.
General Readings
  1. Arnold JN, Wormald MR, Suter DM, Radcliffe CM, Harvey DJ, Dwek RA, et al. Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin. J Biol Chem. 2005 Aug 12;280(32):29080-7. Epub 2005 Jun 14. PubMed PMID: 15955802.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
TRIS buffered Saline containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen.
The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance.
In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
Species:
Human.
Gene ID 3512

Accessory Products

Proteins and/or Positive Controls

Proteins for Immunoglobulin J chain (5 products)

Catalog No. Species Pres. Purity   Source  

Immunoglobulin J chain

Immunoglobulin J chain Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Immunoglobulin J chain (23-159, His-tag)

Immunoglobulin J chain Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

Immunoglobulin J chain (23-159, His-tag)

Immunoglobulin J chain Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

Immunoglobulin J chain

Immunoglobulin J chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Immunoglobulin J chain

Immunoglobulin J chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Immunoglobulin J chain (3 products)

Catalog No. Species Pres. Purity   Source  

IGJ 293T Cell Transient Overexpression Lysate(Denatured)

IGJ 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-IGJ antibody (H00003512-B01) by Western Blots.
  Abnova Taiwan Corp.

IgJ Lysate

Western Blot: IgJ Lysate [NBL1-11879] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IGJ
  Novus Biologicals Inc.

IGJ overexpression lysate

IGJ overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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