DDX0270B-100 Influenza M1/HLA-A2 Complex (Extracell. Dom.) antibody

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0.1 mg / €480.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Influenza M1/HLA-A2 Complex 405H1.01

Product Description for Influenza M1/HLA-A2 Complex

Mouse anti Human Influenza M1/HLA-A2 Complex 405H1.01.
Properties: (Extracell. Dom.)
Presentation: Biotin
Product is tested for Enzyme Immunoassay.

Properties for Influenza M1/HLA-A2 Complex

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Biotin
Reactivity Hu
Applications E
Clonality Monoclonal
Clone 405H1.01
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Immunogen:
T2 cell line charged in M1 peptide
Property Extracell. Dom.
Isotype control SM10B (for use in human samples)
Application DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA.
DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry.
DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. 
DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. 
DDX0270B-50 / DDX0270B-100 Biotin: ELISA.

Usage recommendation:

*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule.
Concentration 0.5 mg/ml
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Biotin
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
Specificity
Specificity:
Human extracellular/M1 HLA neuropilin-1(epitope in extracellular domain).

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