Product Description for Influenza M1 / HLA-A2 Complex
Mouse anti Human Influenza M1 / HLA-A2 Complex 405H1.01.
Properties: (Extracell. Dom.)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.
Properties: (Extracell. Dom.)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.
Properties for Influenza M1 / HLA-A2 Complex
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, F |
| Clonality | Monoclonal |
| Clone | 405H1.01 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | Extracell. Dom. |
| Isotype control | AM03095PU-N |
| Application | DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA. DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry. DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. DDX0270B-50 / DDX0270B-100 Biotin: ELISA. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Background | In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule. |
| Concentration | 0.5 mg/ml |
| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
| Format |
|
| Specificity |
|
Accessory Products
Recommended Secondary Antibodies for Influenza M1 / HLA-A2 Complex (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | AP | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| Acris Antibodies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| Acris Antibodies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| Acris Antibodies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| Acris Antibodies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| Acris Antibodies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| Acris Antibodies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| Acris Antibodies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| Acris Antibodies GmbH | |||||||
Compare Products
You have no items to compare.
My Cart
You have no items in your shopping cart.
Over 280,000 products but you can't find the right antibody for your protein or application?
Acris will do the search for you!
