Product Description for Influenza M1 / HLA-A2 Complex
Mouse anti Human Influenza M1 / HLA-A2 Complex 405H1.01.
Properties: (Extracell. Dom.)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.
Properties: (Extracell. Dom.)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.
Properties for Influenza M1 / HLA-A2 Complex
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | E, F |
| Clonality | Monoclonal |
| Clone | 405H1.01 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Property | Extracell. Dom. |
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | DDX0270P-50 / DDX0270P-100 Purified: Flow cytometry, ELISA. DDX0270A488-50 / DDX0270A488-100 Alexa-fluor®488: Flow cytometry. DDX0270A546-50 / DDX0270A546-100 Alexa-fluor®488: Flow cytometry.. DDX0270A647-50 / DDX0270A647-100 Alexa-fluor®488: Flow cytometry. DDX0270B-50 / DDX0270B-100 Biotin: ELISA. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Background | In order to generate this mAb, the HLA.A2 heavy chain and human 2-m were produced in bacteria, purified and covalently bound to the immunodominant HLA.A2 restricted peptide 58-66 of the influenza virus matrix protein (M1p). Correct refolding was confirmed by staining specific CD8+ CTL clones with the biotinylated recombinant chimera that had been tetramerized with PE-streptavidin. Balb/c mice were immunized with the chimera and hybridomas were tested by a differential chimera-based ELISA containing M1-HLA.A2 versus HIVgag-HLA.A2 and empty HLA.A2 constructs. 405H1 mAb was found to be specific for M1-HLA.A2 with a Kd = 3,3.10-10 M. 405H1 represents a valuable tool for studying the processing and direct or cross-presentation on MHC-I molecule. |
| Concentration | 0.5 mg/ml |
| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
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Accessory Products
Recommended Secondary Antibodies for Influenza M1 / HLA-A2 Complex (11 products)
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| R1253HRP |
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| R1253TR |
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Rabbit | Texas Red | ICC/IF | ||||
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Rabbit | PE | F | ||||
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| R1405R |
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Goat | PE | |||||
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| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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Donkey | PE | |||||
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| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
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Goat | Cy2 | |||||
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| R1457C3 |
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Goat | Cy3 | |||||
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| R1457C5 |
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Goat | Cy5 | |||||
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