discontinued

AP56115PU-N Inscuteable antibody

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0.1 mg / €430.00

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Rabbit anti Human, Rat Inscuteable

AP56115PU-N

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  • AP56115PU-N

Product Description for Inscuteable

Rabbit anti Human, Rat Inscuteable.
Presentation: Purified
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Inscuteable

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Protein inscuteable homolog
Presentation Purified
Reactivity Hu, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~63 kDa observed.Uncharacterized bands may be observed at ~70-85 kDa and ~225 kDA in some cell lysates.
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q1MX18
Immunogen:
KLH-conjugated linear peptide corresponding to the human protein inscuteable homolog.
GeneID:
387755
Application Immunohistochemistry: 2 µg/ml from a representative lot detected Inscuteable in paraffin-embedded human kidney and colon tissues.
Western Blot: A previous lot of this antibody was used to detect Inscuteable in L6 cell lysates.
Background Inscuteable (minsc or protein inscuteable homolog) is known to act as key regulator of spindle orientation in Drosophila. During mammalian neurogenesis, it is expressed temporally and spatially and is thought to influence the orientation of neural progenitor divisions, which are important for cell differentiation in the retina. Aside from its ability to influence the cytoskeletal structure of neural progenitor cells, it is suggested that Gi-alpha and inscuteable may play a role in regulating cellular stress associated with protein-processing pathologies. In particular, inscuteable augments the distribution of AGS3 within the aggresome whereas the AGS3 binding partner, Gi-alpha, is shown to rescue it from the aggresome.
Concentration lot-specific
Protocols Western Blotting
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on cell lysate and transfer the proteins to a PVDF membrane. Wash the PVDF membrane twice with water.
2. Block the blotted PVDF membrane in freshly prepared 5% BSA or milk with 0.05% Tween®-20 surfactant for 1 hour at room temperature with constant agitation.
3. Incubate the PVDF with the recommended dilution of the primary antibody, diluted in freshly prepared 5% BSA or milk for 1 hour at room temperature or overnight with agitation at 2-8°C.
4. Wash the PVDF 3 times with TBST.
5. Incubate the PVDF in the secondary reagent of choice in 5% milk for 1 hour with agitation at room temperature.
6. Wash the PVDF 3-5 times with TBST.
7. Visualize with enhanced chemiluminescence (ECL) method of choice.
General Readings
  1. Zigman M, Cayouette M, Charalambous C, Schleiffer A, Hoeller O, Dunican D, et al. Mammalian inscuteable regulates spindle orientation and cell fate in the developing retina. Neuron. 2005 Nov 23;48(4):539-45. PubMed PMID: 16301171.
  2. Izaki T, Kamakura S, Kohjima M, Sumimoto H. Two forms of human Inscuteable-related protein that links Par3 to the Pins homologues LGN and AGS3. Biochem Biophys Res Commun. 2006 Mar 24;341(4):1001-6. Epub 2006 Jan 24. PubMed PMID: 16458856.
  3. Vural A, Oner S, An N, Simon V, Ma D, Blumer JB, et al. Distribution of activator of G-protein signaling 3 within the aggresomal pathway: role of specific residues in the tetratricopeptide repeat domain and differential regulation by the AGS3 binding partners Gi(alpha) and mammalian inscuteable. Mol Cell Biol. 2010 Mar;30(6):1528-40. doi: 10.1128/MCB.01018-09. Epub 2010 Jan 11. PubMed PMID: 20065032. (Free PMC Article available, 13 images available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A Chromatography
Buffer System:
0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
State:
Liquid purified Ig fraction
Purified
Specificity
Species:
Rat, Human.

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