AM50117PU-T Insulin-like growth factor I / IGF1 antibody

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20 µg / €180.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Mouse, Rabbit, Rat Insulin-like growth factor I / IGF1 SPM406

AM50117PU-T

Product Description for Insulin-like growth factor I / IGF1

Mouse anti Human, Mouse, Rabbit, Rat Insulin-like growth factor I / IGF1 SPM406.
Presentation: Purified
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Insulin-like growth factor I / IGF1

Product Category Primary Antibodies
Target Category
Quantity 20 µg
Synonyms IBP1, IGF-I, MGF, Mechano growth factor, Somatomedin-C
Presentation Purified
Reactivity Hu, Ms, Rb, Rt
Applications F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM406
Host Mouse
Isotype IgG1
Molecular weight ~7.6kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P05019
Immunogen:
Purified Human IGF-1 protein.
GeneID:
3479
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
In-vitro Neutralization of Biological Activity of IGF-1 (Use Azide Free Antibody). 
Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein.
Background IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon
Concentration 0.2 mg/ml
General Readings
  1. Rotwein P, Pollock KM, Didier DK, Krivi GG. Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides. J Biol Chem. 1986 Apr 15;261(11):4828-32. PubMed PMID: 2937782.
  2. Sandberg-Nordqvist AC, Ståhlbom PA, Reinecke M, Collins VP, von Holst H, Sara V. Characterization of insulin-like growth factor 1 in human primary brain tumors. Cancer Res. 1993 Jun 1;53(11):2475-8. PubMed PMID: 8495408.
  3. Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. 
Cellular Localization: Cytoplasmic (Secreted).
Species:
Human, Mouse, Rat, Rabbit.

Accessory Products

Proteins and/or Positive Controls

Proteins for Insulin-like growth factor I / IGF1 (28 products)

Catalog No. Species Pres. Purity   Source  

Insulin Like Growth Factor-I (Des 1-3)

Insulin Like Growth Factor-I (Des 1-3) Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Insulin Like Growth Factor-I Des (1-3)

Insulin Like Growth Factor-I Des (1-3) Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Insulin Like Growth Factor-I Des (1-3)

Insulin Like Growth Factor-I Des (1-3) Human Purified >95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
E. coli
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1 (Long R3)

Insulin-like growth factor I / IGF1 (Long R3) Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
1 mg / €220.00
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1 (Long R3)

Insulin-like growth factor I / IGF1 (Long R3) Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
0.2 mg / €140.00
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1 (transcript variant 4)

Insulin-like growth factor I / IGF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Insulin-like growth factor I / IGF1 (transcript variant 4)

Insulin-like growth factor I / IGF1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
  OriGene Technologies, Inc.

Insulin-like growth factor I / IGF1 (transcript variant 4)

Insulin-like growth factor I / IGF1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

Insulin-like growth factor I / IGF1 (transcript variant 4)

Insulin-like growth factor I / IGF1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
0.1 mg / €139.00
  OriGene Technologies, Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified > 95 % pure by SDS-PAGE E. coli
0.5 mg / €540.00
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified > 95 % pure by SDS-PAGE E. coli
0.1 mg / €230.00
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human > 98 % pure by SDS-PAGE and HPLC analyses. E. coli
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified > 95 % pure as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
E. coli
  OriGene Technologies GmbH

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human
  Abnova Taiwan Corp.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified > 97 % E. coli
50 µg / €250.00
  Neuromics Antibodies

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified > 97 % E. coli
0.25 mg / €640.00
  Neuromics Antibodies

Insulin-like growth factor I / IGF1

SDS-Page: IGF1 Protein [NBC1-18458] - IGF-1, 7.7kDa (71aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified
  Novus Biologicals Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human Purified
  Novus Biologicals Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human > 98 % E. coli
  QED Bioscience Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human > 98 % E. coli
  QED Bioscience Inc.

Insulin-like growth factor I / IGF1

Insulin-like growth factor I / IGF1 Human > 98 % E. coli
  QED Bioscience Inc.

Positive controls for Insulin-like growth factor I / IGF1 (2 products)

Catalog No. Species Pres. Purity   Source  

IGF1 Lysate

Western Blot: IGF1 Lysate [NBL1-11866] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IGF1
  Novus Biologicals Inc.

IGF1 overexpression lysate

IGF1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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