NSB872 Integrin beta 1 Phosphospecific antibody

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Rabbit anti Human Integrin beta 1 Phosphospecific

Product Description for Integrin beta 1 Phosphospecific

Rabbit anti Human Integrin beta 1 Phosphospecific.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Integrin beta 1 Phosphospecific

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558). The sequence is conserved in human, mouse, rat and chicken.
Add. information Research Areas: Neuroscience, Stem Cell Markers, Immunology
Application Western Blot, Western Blot Predicted molecular weight 130 kDa. , Western Blot 1:1,000
Background Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Concentration 0.5 mg/ml
General Readings General / background references:Blystone SD et al. A molecular mechanism of integrin crosstalk: alphavbeta3 suppression of calcium/calmodulin-dependent protein kinase II regulates alpha5beta1 function. J Cell Biol 145:889-97 (1999). PubMed PMID: 10330414
Schlaepfer DD et al. Signaling through focal adhesion kinase. Prog Biophys Mol Biol 71:435-78 (1999). PubMed PMID: 10354709
Wennerberg K et al. Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin beta1A. Requirement for threonines 788-789 in receptor activation. J Cell Sci 111 ( Pt 8):1117-26 (1998). PubMed PMID: 9512507
Richardson A & Parsons JT Signal transduction through integrins: a central role for focal adhesion kinase? Bioessays 17:229-36 (1995). PubMed PMID: 7748177
5. Schiless, F., et al. Involvement of integrins and Src in insulin signaling toward autophagic proteolysis in rat liver. J. Biol. Chem. 279(20):21294-21301, 2004.
6. Liu, B.P., & Burridge, K. Vav2 activates Rac1, Cdc42, and RhoA downstream from growth factor receptors but not beta1 integrins. Mol. Cell Biol. 20(19):7160-7169, 2000.
7. Arthur, W.T., et al. Integrin engagement suppresses RhoA activity via c-Src-dependent mechanism. Curr. Biol. 10(12):719-722, 2000.
8. Schlaepfer, D.D., Hunter, T. Integrin signalling and tyrosine phosphrylation:just the FAKs? Trends Cell Biol. 8(4):151-157, 1998.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
Integrin beta 1 Phosphospecific [Thr788/Thr789]

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